编码主要屋尘螨变应原Der p 1的cDNA序列分析。与半胱氨酸蛋白酶的同源性。
Sequence analysis of cDNA coding for a major house dust mite allergen, Der p 1. Homology with cysteine proteases.
作者信息
Chua K Y, Stewart G A, Thomas W R, Simpson R J, Dilworth R J, Plozza T M, Turner K J
机构信息
Clinical Immunology Research Unit, Princess Margaret Hospital for Children, Subiaco, Western Australia.
出版信息
J Exp Med. 1988 Jan 1;167(1):175-82. doi: 10.1084/jem.167.1.175.
Abstract
A cDNA clone coding for Der p 1, a major allergen from the house dust mite Dermatophagoides pteronyssinus, has been sequenced. It codes for a 222 residue mature protein with a derived molecular weight of 25,371 and contains 1 potential N-glycosylation site. In addition, the cDNA appears to code for a 13 residue proregion, and an incomplete signal peptide. The deduced sequence shows a high degree of homology with animal and plant cysteine proteases, particularly in the region of the contact residues making up the active site. Southern analysis of genomic DNA indicates that the allergen is coded by a noncontiguous gene. These data will now facilitate epitope mapping studies.
摘要
编码来自屋尘螨（粉尘螨）主要变应原Der p 1的一个cDNA克隆已被测序。它编码一个含222个残基的成熟蛋白，推导的分子量为25371，且含有1个潜在的N - 糖基化位点。此外，该cDNA似乎编码一个含13个残基的前区域和一个不完整的信号肽。推导的序列与动植物半胱氨酸蛋白酶显示出高度同源性，尤其是在构成活性位点的接触残基区域。基因组DNA的Southern分析表明该变应原由一个不连续的基因编码。这些数据现在将有助于表位作图研究。
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