人类单克隆丙种球蛋白病中淋巴细胞的独特型特异性：用荧光激活细胞分选仪进行分析
The idiotypic specificities of lymphocytes in human monoclonal gammopathies: analysis with the fluorescence activated cell sorter.
作者信息
Carmagnola A L, Boccadoro M, Massaia M, Pileri A
出版信息
Clin Exp Immunol. 1983 Jan;51(1):173-7.
Four monoclonal IgG kappa gammopathies were studied with anti-idiotypic sera and the percentage of lymphocytes bearing such idiotypes was evaluated with a fluorescence activated cell sorter (FACS). A large percentage (10-15%) of peripheral lymphocytes bearing receptors with the same idiotypic specificities as the M component was observed in multiple myeloma (MM) and in monoclonal gammopathy of undetermined significance (MGUS), suggesting a malignant origin for both these diseases. Capping-endocytosis and resynthesis experiments showed that these idiotypic receptors were not simply adsorbed on the cell membrane, but actually synthesized by the cells bearing them. Experiments performed after effective conventional chemotherapy showed a strong reduction of the idiotypic compartment in only one MM patient.
利用抗独特型血清对4种单克隆IgG κ型丙种球蛋白病进行了研究，并用荧光激活细胞分选仪（FACS）评估了携带此类独特型的淋巴细胞百分比。在多发性骨髓瘤（MM）和意义未明的单克隆丙种球蛋白病（MGUS）中，观察到很大比例（10 - 15%）的外周淋巴细胞带有与M成分具有相同独特型特异性的受体，这表明这两种疾病均起源于恶性病变。封帽 - 内吞和再合成实验表明，这些独特型受体并非简单地吸附在细胞膜上，而是由携带它们的细胞实际合成的。在有效的传统化疗后进行的实验显示，仅一名MM患者的独特型区室有显著减少。
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