Mellstedt H, Pettersson D, Holm G
Scand J Haematol. 1976 Feb;16(2):112-20. doi: 10.1111/j.1600-0609.1976.tb01125.x.
Antisera were produced in rabbits against idiotypic determinants of the myeloma protein from 3 patients with IgG-k myeloma. The antisera did neither cross-react nor react with normal immunoglobulin (Ig). Idiotypic Ig-structures were demonstrated by indirect immunofluorescence (IFL) on 70-94% of peripheral B-lymphocytes inuntreated patients. The total numbers of circulating B-lymphocytes were increased with a simultaneous decrease of T-lymphocytes. Only one of the patients had blood lymphocytes which stained for cytoplasmic Ig with idiotypic antiserum. The monoclonal lymphocyte population decreased gradually during treatment. Plasma cell counts in bone marrow, IgG concentration in serum and erythrocyte sedimentation rate decreased simultaneously, whilt the haemoglobin concentration increased. Very few monoclonal lymphocytes were present during remission. Later, the monoclonal lymphocyte population began to increase in 2 of the patients in parallel with clinical signs indicating relapse. It is concluded that presence of monoclonal B-lymphocytes indicates dissemination of the disease and that they may belong to the malignant cell clone.
用来自3例IgG-κ型骨髓瘤患者的骨髓瘤蛋白的独特型决定簇免疫家兔制备抗血清。这些抗血清既不发生交叉反应，也不与正常免疫球蛋白（Ig）发生反应。通过间接免疫荧光法（IFL）在未经治疗患者70% - 94%的外周B淋巴细胞上证实了独特型Ig结构。循环B淋巴细胞总数增加，同时T淋巴细胞减少。仅1例患者的血淋巴细胞用独特型抗血清对细胞质Ig呈阳性染色。治疗期间单克隆淋巴细胞群体逐渐减少。骨髓中的浆细胞计数、血清IgG浓度和红细胞沉降率同时下降，而血红蛋白浓度升高。缓解期很少有单克隆淋巴细胞。后来，2例患者的单克隆淋巴细胞群体开始增加，同时出现提示复发的临床体征。结论是单克隆B淋巴细胞的存在表明疾病已播散，并且它们可能属于恶性细胞克隆。
