Tabor E, Purcell R H, London W T, Gerety R J
J Infect Dis. 1983 Mar;147(3):531-4. doi: 10.1093/infdis/147.3.531.
The infectivity of three inocula (subtypes ayw, adr, and adw) of hepatitis B virus was evaluated by intravenous inoculation of 54 chimpanzees. End-point infectivity titers were 10(-7.5) (ayw), 10(-8.0) (adr), and 10(-7.0) (adw). In contrast, the end-point titers for detection of hepatitis B surface antigen by radioimmunoassay were 10(-4) (ayw), 10(-4) (adr), and 10(-5) (adw). The mean incubation period for infections transmitted by each dilution of each of the inocula was inversely proportional to the amount of infectious virus in the dilution, but substantial overlap was observed among proximate dilutions. The severity of hepatitis in the chimpanzees differed among the inocula, but it was unrelated to the amount of virus inoculated. Thus, conclusions about the infectivity of hepatitis B virus after inactivation based on titers of hepatitis B surface antigen or on the inverse relationship between the incubation period and the end-point infectivity titer must be made with caution.
通过对54只黑猩猩进行静脉接种，评估了三种乙肝病毒接种物（ayw、adr和adw亚型）的传染性。终点感染滴度分别为10^(-7.5)（ayw）、10^(-8.0)（adr）和10^(-7.0)（adw）。相比之下，通过放射免疫测定法检测乙肝表面抗原的终点滴度分别为10^(-4)（ayw）、10^(-4)（adr）和10^(-5)（adw）。每种接种物的每种稀释度所传播感染的平均潜伏期与稀释液中传染性病毒的量成反比，但在相近稀释度之间观察到大量重叠。黑猩猩中肝炎的严重程度在接种物之间有所不同，但与接种的病毒量无关。因此，基于乙肝表面抗原滴度或潜伏期与终点感染滴度之间的反比关系来推断乙肝病毒灭活后的传染性时，必须谨慎得出结论。
