Thomas T J, Bloomfield V A
Nucleic Acids Res. 1983 Mar 25;11(6):1919-30. doi: 10.1093/nar/11.6.1919.
The solution properties of the B and Z forms of poly(dG-dC).poly(dG-dC) have been measured by static and dynamic laser light scattering. The radius of gyration, persistence length, translational and segmental diffusion coefficients, and the Rouse-Zimm parameters have been evaluated. The persistence length of the Z form determined at 3 M NaCl is about 200 nm compared to 84 and 61 nm respectively for the B forms of poly(dG-dC).poly(dG-dC), and calf thymus DNA, both determined at 0.1 M NaCl. The data on persistence length, diffusion coefficients and the Rouse-Zimm parameters indicate a large increase in the chain stiffness of Z DNA compared to the B form. These results are opposite to the ionic strength effects on random sequence native DNAs, for which the flexibility increases with ionic strength and levels off at about 1 M NaCl.
通过静态和动态激光光散射测量了聚（dG-dC）·聚（dG-dC）的B型和Z型的溶液性质。评估了回转半径、持久长度、平移和链段扩散系数以及劳斯-齐姆参数。在3M NaCl条件下测定的Z型的持久长度约为200nm，而在0.1M NaCl条件下测定的聚（dG-dC）·聚（dG-dC）的B型和小牛胸腺DNA的持久长度分别为84nm和61nm。关于持久长度、扩散系数和劳斯-齐姆参数的数据表明，与B型相比，Z型DNA的链刚性大幅增加。这些结果与离子强度对随机序列天然DNA的影响相反，对于随机序列天然DNA，其柔韧性随离子强度增加，并在约1M NaCl时趋于平稳。
