六种植物丙酮提取物的艾姆斯诱变试验评估。

An evaluation of acetone extracts from six plants in the Ames mutagenicity test.

作者信息

White R D, Krumperman P H, Cheeke P R, Buhler D R

出版信息

Toxicol Lett. 1983 Jan;15(1):25-31. doi: 10.1016/0378-4274(83)90164-9.

Abstract

Acetone extracts from six plants were evaluated for mutagenic activity with the Salmonella/mammalian-microsome mutagenicity test (Ames) utilizing tester strains TA98 and TA100 and in the presence and absence of induced rat liver microsomes. Extracts from alfalfa (Medicago sativa), lettuce (Lactuca sativa) and thread-leaf groundsel (Senecio longilobus) produced only negative responses. Comfrey (Symphytum officinale) and tansy ragwort (Senecio jacobaea) extracts produced toxic responses that were abolished in the presence of the microsomal bioactivation system. Bracken (Pteridium aquilinum) and tansy ragwort extracts produced positive responses following bioactivation with the liver microsomal system. The results suggest that the Ames mutagenicity test may be of some value in initial evaluations for potential toxic effects of plants consumed by animals and man.

摘要

利用鼠伤寒沙门氏菌/哺乳动物微粒体诱变性试验（艾姆斯试验），使用测试菌株TA98和TA100，在有和没有诱导大鼠肝微粒体的情况下，对六种植物的丙酮提取物进行了诱变活性评估。紫花苜蓿（Medicago sativa）、生菜（Lactuca sativa）和线叶千里光（Senecio longilobus）的提取物仅产生阴性反应。紫草（Symphytum officinale）和欧洲千里光（Senecio jacobaea）的提取物产生了毒性反应，在微粒体生物活化系统存在的情况下这种反应消失了。蕨菜（Pteridium aquilinum）和欧洲千里光的提取物在经肝微粒体系统生物活化后产生了阳性反应。结果表明，艾姆斯诱变性试验在初步评估人和动物食用的植物的潜在毒性作用方面可能具有一定价值。