Collins M T, Cho S N, Høiby N, Espersen F, Baek L, Reif J S
Infect Immun. 1983 Mar;39(3):1428-40. doi: 10.1128/iai.39.3.1428-1440.1983.
By crossed immunoelectrophoresis, 85 different antigens were demonstrated in sonicated preparations of Legionella pneumophila serogroup 1 (Lp1). The precipitin patterns of 82 anodic-migrating antigens were numbered and were designated the Lp1 reference system. Eleven antigens were stable to boiling, and seven of these were shown to be surface antigens. One heat-stable surface antigen (antigen no. 61) was highly reactive with limulus amoebocyte lysates and formed a precipitin resembling lipopolysaccharide. Serum from an isolation confirmed case of Lp1 infection and serogroup-specific rabbit antiserum reacted specifically with antigen no. 61, which was designated the serogroup-specific antigen. Normal human and rabbit sera commonly had antibodies to antigen no. 66 of the Lp1 reference system. This antigen is antigenically related to the "common antigen" of Pseudomonas aeruginosa.
通过交叉免疫电泳，在嗜肺军团菌血清1型（Lp1）的超声破碎制剂中证实了85种不同的抗原。对82种向阳极迁移的抗原的沉淀模式进行了编号，并将其指定为Lp1参考系统。11种抗原对煮沸稳定，其中7种被证明是表面抗原。一种热稳定表面抗原（抗原编号61）与鲎变形细胞溶解物高度反应，并形成类似于脂多糖的沉淀素。来自一例确诊的Lp1感染病例的分离血清和血清群特异性兔抗血清与抗原编号61特异性反应，该抗原被指定为血清群特异性抗原。正常人和兔血清通常含有针对Lp1参考系统抗原编号66的抗体。该抗原在抗原性上与铜绿假单胞菌的“共同抗原”相关。
