Joly J R, Kenny G E
Infect Immun. 1982 Feb;35(2):721-9. doi: 10.1128/iai.35.2.721-729.1982.
The antigens of the six serogroups of Legionella pneumophila were compared by two-dimensional (crossed) immunoelectrophoresis by using rabbit antisera to serogroups 1, 2, 3 and 4. The close relationship among the serogroups was shown by the fact that 27 of the 31 antigens demonstrated so far were common. However, distinctive group-specific antigens with slow electrophoretic mobility were observed for serogroups 1, 2, 3, and 4. When intact serogroup 1 organisms were extracted with EDTA, the group-specific antigen was recovered in a virtually pure form. The group-specific antigen was pronase resistant, heat stable, and amphiphilic and had a surface location, all of which are properties suggestive of lipopolysaccharide. L. pneumophila shared four to five antigens with Tatlockia micdadei (Legionella micdadei). The large number of common antigens in the serogroups of L. pneumophila has important implications for the specific detection of antigens and antibodies by fluorescent and other tagged antibody methods.
利用针对嗜肺军团菌血清群1、2、3和4的兔抗血清，通过二维（交叉）免疫电泳对嗜肺军团菌六个血清群的抗原进行了比较。血清群之间的密切关系表现为，迄今已证实的31种抗原中有27种是共同的。然而，在血清群1、2、3和4中观察到了具有缓慢电泳迁移率的独特的群特异性抗原。当用EDTA提取完整的血清群1生物体时，群特异性抗原以几乎纯的形式被回收。该群特异性抗原对链霉蛋白酶有抗性，耐热且具有两亲性，并且位于表面，所有这些特性都表明它是脂多糖。嗜肺军团菌与米克戴德军团菌（嗜肺军团菌米克戴德亚种）共有四到五种抗原。嗜肺军团菌血清群中大量的共同抗原对于通过荧光和其他标记抗体方法进行抗原和抗体的特异性检测具有重要意义。
