Kuopio T, Pelliniemi L J
Department of Anatomy, University of Turku, Finland.
Cell Tissue Res. 1989 Apr;256(1):45-51. doi: 10.1007/BF00224717.
Rat testes were examined by conventional and immunolabeling transmission electron microscopy. Ultrastructurally identifiable continuous basement membranes were found around seminiferous tubules and the interstitial capillaries. Patches of basement membrane were, additionally, found on free surfaces of Leydig cells, between two Leydig cells, and in macrophage-Leydig cell contact sites. The ultrastructural findings were confirmed by immunocytochemical localization of laminin and collagen type IV in the same areas. A close association between the capillary basement membranes and the surfaces of perivascular Leydig cells was also observed. The possible basement membrane-mediated interactions of Leydig cells with other testicular structures, together with the novel bioactive products and regulators of Leydig cells, support the role of these cells as exceptionally complex regulatory centers of testicular functions.
通过传统和免疫标记透射电子显微镜对大鼠睾丸进行检查。在生精小管和间质毛细血管周围发现了超微结构可识别的连续基底膜。此外，在睾丸间质细胞的游离表面、两个睾丸间质细胞之间以及巨噬细胞-睾丸间质细胞接触部位发现了基底膜斑块。通过在相同区域对层粘连蛋白和IV型胶原进行免疫细胞化学定位，证实了超微结构的发现。还观察到毛细血管基底膜与血管周围睾丸间质细胞表面之间存在密切关联。睾丸间质细胞与其他睾丸结构之间可能通过基底膜介导的相互作用，以及睾丸间质细胞的新型生物活性产物和调节因子，支持了这些细胞作为睾丸功能异常复杂调节中心的作用。
