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5型腺病毒与12型腺病毒早期区域1A之间非缺陷重组体的分离

Isolation of a nondefective recombinant between adenovirus type 5 and early region 1A of adenovirus type 12.

作者信息

Shiroki K, Saito I, Maruyama K, Shimojo H

出版信息

J Virol. 1983 May;46(2):632-7. doi: 10.1128/JVI.46.2.632-637.1983.

DOI:10.1128/JVI.46.2.632-637.1983
PMID:6842682
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC255168/
Abstract

A nondefective recombinant between adenovirus type 5 (Ad5) and type 12 (Ad12), rc-1 (Ad5 dl312, carrying the Ad12 E1A gene), was isolated from hamster cell foci transformed by a defective recombinant, rcB-1 (dl312, carrying the Ad12 E1 gene). The recombinant rc-1 grew in human embryo kidney and KB cells in the absence of helper and synthesized Ad12 T antigen g, the product of the E1A gene. The genome of rc-1 has a deletion between 79.9 and 82.5 map units of Ad5 dl312 DNA with an insertion of 0.1 to 5.5 map units of Ad12 DNA at the deletion site. The mRNAs of Ad12 E1A were transcribed from the Ad12 E1A promoter, and unusual RNAs were abundantly transcribed from the Ad5 E3 promoter on the opposite strand. The frequency of cell transformation with rc-1 was lower than those with Ad5 and Ad12 wild types.

摘要

从由缺陷重组体rcB-1（dl312，携带腺病毒12型（Ad12）E1基因）转化的仓鼠细胞灶中分离出腺病毒5型（Ad5）和12型（Ad12）之间的无缺陷重组体rc-1（Ad5 dl312，携带Ad12 E1A基因）。重组体rc-1在无辅助病毒的情况下能在人胚肾细胞和KB细胞中生长，并合成Ad12 T抗原g，即E1A基因的产物。rc-1的基因组在Ad5 dl312 DNA的79.9至82.5图谱单位之间有一个缺失，在缺失位点插入了0.1至5.5图谱单位的Ad12 DNA。Ad12 E1A的mRNA从Ad12 E1A启动子转录而来，相反链上的Ad5 E3启动子大量转录出异常RNA。rc-1诱导细胞转化的频率低于Ad5和Ad12野生型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06f8/255168/15b5ec07aa3e/jvirol00146-0311-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06f8/255168/177b412a4da9/jvirol00146-0310-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06f8/255168/15b5ec07aa3e/jvirol00146-0311-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06f8/255168/177b412a4da9/jvirol00146-0310-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06f8/255168/15b5ec07aa3e/jvirol00146-0311-a.jpg

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Isolation of a nondefective recombinant between adenovirus type 5 and early region 1A of adenovirus type 12.5型腺病毒与12型腺病毒早期区域1A之间非缺陷重组体的分离
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引用本文的文献

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Construction of nondefective adenovirus type 5 bearing a 2.8-kilobase hepatitis B virus DNA near the right end of its genome.在其基因组右端附近携带2.8千碱基乙肝病毒DNA的无缺陷5型腺病毒的构建。
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Activation of the human beta interferon gene by the adenovirus type 12 E1B gene.

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The quest for human cancer viruses.对人类癌症病毒的探索。
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Complementation of adenovirus type 5 host range mutants by adenovirus type 12 in coinfected HeLa and BHK-21 cells.腺病毒12型在共感染的HeLa和BHK-21细胞中对腺病毒5型宿主范围突变体的互补作用。
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Evidence for functional relatedness of products encoded by the transforming sequences of human adenovirus types 5 and 12.人5型和12型腺病毒转化序列所编码产物功能相关性的证据。
Virology. 1981 Apr 15;110(1):208-12. doi: 10.1016/0042-6822(81)90023-4.
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Characterization of cells transformed by Ad5/Ad12 hybrid early region I plasmids.由Ad5/Ad12杂交早期区域I质粒转化的细胞的特性分析。
Virology. 1982 Jul 30;120(2):422-32. doi: 10.1016/0042-6822(82)90042-3.
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Dependence of tumor-forming capacities of cells transformed by recombinants between adenovirus types 5 and 12 on expression of early region 1.5型腺病毒与12型腺病毒重组体转化细胞的致瘤能力对早期区域1表达的依赖性
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8
A study to determine the reasons for differences in the tumorigenicity of rat cell lines transformed by adenovirus 2 and adenovirus 12.一项旨在确定由腺病毒2型和腺病毒12型转化的大鼠细胞系致瘤性差异原因的研究。
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Expression of adenovirus type 12 early region 1 in KB cells transformed by recombinants containing the gene.12型腺病毒早期区域1在含该基因的重组体转化的KB细胞中的表达
J Virol. 1983 Mar;45(3):1074-82. doi: 10.1128/JVI.45.3.1074-1082.1983.
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Mapping of RNAs transcribed from adenovirus type 12 early and VA RNA regions.腺病毒12型早期区域和VA RNA区域转录的RNA图谱分析
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