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用于估计大角羊原圆线虫感染情况的贝尔曼技术:实验室操作的影响

The Baermann technique for estimating Protostrongylus infection in bighorn sheep: effect of laboratory procedures.

作者信息

Beane R D, Hobbs N T

出版信息

J Wildl Dis. 1983 Jan;19(1):7-9. doi: 10.7589/0090-3558-19.1.7.

DOI:10.7589/0090-3558-19.1.7
PMID:6842742
Abstract

The modified Baermann funnel technique was evaluated to determine the effects of time of baermannization, fecal preparation, type and size of funnel, and type of filter on the number of first stage larvae of Protostrongylus spp. recovered from feces of Rocky Mountain bighorn sheep (Ovis canadensis). More larvae were recovered when fecal pellets were baermannized for 24 hr compared to 8 hr, and when feces were crushed than when left intact. Use of small funnels resulted in the recovery of more larvae per gram of feces than larger funnels, and glass funnels more than plastic ones. There was no difference in recovery of larvae between cheesecloth filters and cellulose filters.

摘要

对改良的贝尔曼漏斗技术进行了评估,以确定贝尔曼化时间、粪便制备、漏斗类型和尺寸以及过滤器类型对从落基山大角羊(加拿大盘羊)粪便中回收的原圆线虫属第一期幼虫数量的影响。与8小时相比,粪便颗粒进行24小时贝尔曼化时回收的幼虫更多,粪便被碾碎时比完整时回收的幼虫更多。使用小漏斗每克粪便回收的幼虫比大漏斗多,玻璃漏斗比塑料漏斗回收的幼虫多。粗棉布过滤器和纤维素过滤器在幼虫回收方面没有差异。

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