嗜温性纤维素分解梭菌H10菌株对葡萄糖和纤维二糖的代谢
Metabolism of glucose and cellobiose by cellulolytic mesophilic Clostridium sp. strain H10.
作者信息
Giallo J, Gaudin C, Belaich J P, Petitdemange E, Caillet-Mangin F
出版信息
Appl Environ Microbiol. 1983 Mar;45(3):843-9. doi: 10.1128/aem.45.3.843-849.1983.
Abstract
The metabolism of strain H10, a cellulolytic mesophilic Clostridium sp., was studied on glucose and cellobiose as energy and carbon sources. The main products of fermentation of both sugars were acetate, lactate, and ethanol. At low sugar levels, molar growth yields were better for cellobiose than for glucose. In both cases, an inhibition of growth was observed between 1 and 2 g/liter and a total inhibition after the latter concentration. Inhibition was not the result of low pH due to acid formation; growth under static pH conditions was limited in the same way. On the other hand, acetate and lactate had no inhibitory effect when added at concentrations equal to the final titers. Concomitant with the inhibition of growth was a change in metabolic pathways for sugar concentrations between 1 and 2 g/liter, i.e., the production of lactate was higher. After complete inhibition of growth, an accumulation of carbohydrates which were neither glucose nor cellobiose was observed.
摘要
对嗜温性纤维素分解梭菌属菌株H10以葡萄糖和纤维二糖作为能量及碳源时的代谢情况进行了研究。两种糖发酵的主要产物都是乙酸盐、乳酸盐和乙醇。在低糖水平下，纤维二糖的摩尔生长产率比葡萄糖更好。在这两种情况下，在1至2克/升之间观察到生长受到抑制，在该浓度之后完全抑制生长。抑制并非是由于产酸导致的低pH值造成的；在静态pH条件下生长同样受到限制。另一方面，当以等于最终滴度的浓度添加时，乙酸盐和乳酸盐没有抑制作用。与生长抑制同时发生的是，当糖浓度在1至2克/升之间时代谢途径发生了变化，即乳酸盐的产量更高。在生长完全抑制后，观察到了既不是葡萄糖也不是纤维二糖的碳水化合物的积累。
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