Hamann S R, McAllister R G
Clin Chem. 1983 Jan;29(1):158-60.
In this method for measuring nifedipine, a calcium-channel inhibitor now widely used in the treatment of cardiovascular disease, the drug is extracted from plasma under basic conditions into toluene, and an aliquot of the extract is injected directly into a gas chromatograph equipped with an OV-101 column and an electron-capture detector. The standard curve is linear between 1 and 100 micrograms/L; the assay measures not only nifedipine, but also a major metabolic product (found only after oral administration of the parent drug) and photodegradation products. The procedure is rapid and adequately sensitive for routine use in clinical monitoring of nifedipine in plasma.
在这种用于测定硝苯地平（一种目前广泛用于治疗心血管疾病的钙通道抑制剂）的方法中，该药物在碱性条件下从血浆中萃取到甲苯中，然后取一份萃取液直接注入配备有OV - 101柱和电子捕获检测器的气相色谱仪中。标准曲线在1至100微克/升之间呈线性；该测定法不仅能检测硝苯地平，还能检测一种主要代谢产物（仅在口服母体药物后才会出现）和光降解产物。该方法快速且灵敏度足以用于血浆中硝苯地平临床监测的常规检测。
