Perez H D, Horn J K, Ong R, Goldstein I M
J Lab Clin Med. 1983 Jan;101(1):123-9.
Samples of serum and plasma obtained from eight patients with acute pancreatitis were examined for the presence of complement-derived chemotactic activity for human PMN. Significant chemotactic activity was found in acute phase serum and plasma samples from five patients. The presence of chemotactic activity was associated with reduced levels of CH50 as well as degradation products of C5 (detected by a new method, i.e., radioimmunoelectrophoresis). The chemotactic activity was heat-stable (56 degrees C for 30 min), inhibitable by treatment with antibodies to human C5, and exhibited an apparent molecular weight of 16,000 (determined by chromatography on Sephadex G-75). These properties are identical with those of chemotactic C5-derived peptides (C5a and/or C5a des Arg). Recently, C5-derived peptides have been implicated as being mediators of acute lung injury (i.e., "shock lung") in some clinical situations. It is intriguing to speculate, therefore, that circulating C5-derived chemotactic peptides may play a role in the pathogenesis of the lung injury observed in some patients during the course of acute pancreatitis.
对8例急性胰腺炎患者的血清和血浆样本进行检测，以确定是否存在对人中性粒细胞具有趋化活性的补体衍生物质。在5例患者的急性期血清和血浆样本中发现了显著的趋化活性。趋化活性的存在与CH50水平降低以及C5降解产物（通过一种新方法即放射免疫电泳检测）有关。趋化活性具有热稳定性（56℃30分钟），可被抗人C5抗体处理所抑制，其表观分子量为16,000（通过Sephadex G - 75柱层析测定）。这些特性与趋化性C5衍生肽（C5a和/或C5a去精氨酸）的特性相同。最近，在某些临床情况下，C5衍生肽被认为是急性肺损伤（即“休克肺”）的介质。因此，有趣的是推测循环中的C5衍生趋化肽可能在急性胰腺炎病程中某些患者所观察到的肺损伤发病机制中起作用。
