Westrum L E, Gray E G, Burgoyne R D, Barron J
Cell Tissue Res. 1983;231(1):93-102. doi: 10.1007/BF00215777.
Using techniques for enhanced microtubular preservation (albumin pretreatment of Gray) occipital and pyriform cortices of rats were studied by electron microscopy at various stages of development. A close structural relationship was seen between microtubules (mts), focal membrane densities, synaptic vesicles, and presynaptic dense projections (pre-dps) during maturation. Mts were seen in the neonates to be focused onto the inner surface of immature axonic profiles merging or sometimes fragmenting there. Focal densities occur at these attachment sites and thereafter synaptic vesicles clothe the mts and abut onto these presumed primordial pre-dps. Thus, mts may contribute to the initial formation of pre-dps as well as their maintenance in the adult and may act to channel the first synaptic vesicles to the site of eventual synaptic contact and transmitter release.
运用增强微管保存的技术（格雷白蛋白预处理），通过电子显微镜在不同发育阶段对大鼠枕叶和梨状皮质进行了研究。在成熟过程中，微管（mts）、局灶性膜密度、突触小泡和突触前致密突起（pre-dps）之间存在密切的结构关系。在新生儿中，可见微管聚焦于未成熟轴突轮廓的内表面，在那里融合或有时断裂。这些附着部位出现局灶性密度，此后突触小泡覆盖微管并邻接这些推测的原始突触前致密突起。因此，微管可能有助于突触前致密突起在成体中的初始形成及其维持，并且可能起到将第一批突触小泡引导至最终突触接触和递质释放部位的作用。
