Tangerman A, Meuwese-Arends M T, van Tongeren J H
Clin Chim Acta. 1983 May 9;130(1):103-10. doi: 10.1016/0009-8981(83)90263-2.
A new analytical technique is described for measuring volatile sulphur compounds in human breath. The sulphur compounds were trapped and concentrated onto Tenax GC and then assayed by gas chromatography, using a specific sulphur detector. The detection limit amounts to about 0.2 ng/l (0.1 ppb). Among the sulphur volatiles, dimethylsulphide and methanethiol were quantitatively analysed in 100 ml of breath of 20 normal subjects and 35 cirrhotic patients. Dimethylsulphide in the breath of cirrhotics (113.4 +/- 31.9 ng/l, mean +/- SEM) was significantly elevated (p less than 0.05) compared with normals (21.1 +/- 1.7 ng/l). The concentration of methanethiol in the breath of normals and of most cirrhotics was less than 1 ng/l. In only five cirrhotics could methanethiol be detected in 100 ml of breath (3-23 ng/l). Dimethyldisulphide and hydrogen sulphide were not present in detectable amounts in the breath of normals. In cirrhotics, dimethyldisulphide was detected in a few cases. Ethanethiol was absent in the breath of both normals and cirrhotics.
本文描述了一种用于测量人体呼出气体中挥发性硫化合物的新分析技术。硫化合物被捕获并浓缩在Tenax GC上，然后使用特定的硫检测器通过气相色谱法进行分析。检测限约为0.2 ng/l（0.1 ppb）。在20名正常受试者和35名肝硬化患者的100 ml呼出气体中，对挥发性硫化合物中的二甲基硫和甲硫醇进行了定量分析。肝硬化患者呼出气体中的二甲基硫（113.4±31.9 ng/l，平均值±标准误）与正常人（21.1±1.7 ng/l）相比显著升高（p<0.05）。正常人和大多数肝硬化患者呼出气体中甲硫醇的浓度低于1 ng/l。仅在5名肝硬化患者的100 ml呼出气体中检测到甲硫醇（3 - 23 ng/l）。正常人呼出气体中未检测到二甲基二硫和硫化氢。在少数肝硬化患者中检测到了二甲基二硫。正常人和肝硬化患者呼出气体中均未检测到乙硫醇。
