Patchen M L, MacVittie T J
Immunopharmacology. 1983 Apr;5(4):303-13. doi: 10.1016/0162-3109(83)90046-2.
The hemopoietic effects produced by six different doses of a commercially available glucan preparation were investigated. C3H/HeN mice were intravenously injected with either 0.1, 0.4, 0.8, 1.2, 1.6, or 2.0 mg of glucan. Five days later, total nucleated cellularity, pluripotent stem cells (CFU-s), granulocyte-macrophage colony-forming cells (GM-CFC), macrophage colony-forming cells (M-CFC), and erythroid burst- and colony-forming cells (BFU-e and CFU-e) were assayed in the bone marrow and spleen. Neither femoral cellularity nor GM-CFC content was altered by any of the glucan doses tested. By contrast, bone marrow CFU-s content increased and CFU-e and BFU-e contents decreased with increasing glucan doses. In the spleen, all aspects of hemopoiesis (i.e., cellularity, CFU-s, GM-CFC, M-CFC, and CFU-e) increased after glucan administration. The degree of splenic enhancement was directly correlated with the dose of glucan administered.
研究了市售葡聚糖制剂的六种不同剂量所产生的造血作用。给C3H/HeN小鼠静脉注射0.1、0.4、0.8、1.2、1.6或2.0毫克葡聚糖。五天后，对骨髓和脾脏中的总核细胞数、多能干细胞（CFU-s）、粒细胞-巨噬细胞集落形成细胞（GM-CFC）、巨噬细胞集落形成细胞（M-CFC）以及红系爆式集落形成细胞和集落形成细胞（BFU-e和CFU-e）进行了测定。所测试的任何葡聚糖剂量均未改变股骨细胞数或GM-CFC含量。相比之下，随着葡聚糖剂量的增加，骨髓CFU-s含量增加，CFU-e和BFU-e含量降低。在脾脏中，葡聚糖给药后造血的各个方面（即细胞数、CFU-s、GM-CFC、M-CFC和CFU-e）均增加。脾脏增强的程度与所给予的葡聚糖剂量直接相关。
