Patchen M L, MacVittie T J
Int J Immunopharmacol. 1985;7(6):923-32. doi: 10.1016/0192-0561(85)90056-6.
Hemopoietic effects of the reticuloendothelial agent glucan were assayed in normal mice and in mice hemopoietically depleted by exposure to 60Co radiation. In normal mice, glucan administration increased the content of bone marrow and splenic transplantable pluripotent hemopoietic stem cells (CFU-s), committed granulocyte-macrophage progenitor cells (GM-CFC), and pure macrophage progenitor cells (M-CFC). Erythroid progenitor cells (CFU-e) were increased only in the spleen. In sublethally irradiated mice (650 rads), glucan increased the number of endogenous pluripotent hemopoietic stem cells (E-CFU) when administered either before or after irradiation. The most pronounced effects were observed when glucan was administered 1 day before, 1 h before, or 1 h after irradiation. In addition, the administration of glucan before lethal irradiation (900 rads) enhanced survival. The most significant results were seen when glucan was administered 1 day prior to irradiation. The possibility of using agents such as glucan to enhance hemopoietic reconstitution and prevent septicemia following chemotherapy and/or radiotherapy is discussed.
在正常小鼠以及经60Co辐射造成造血功能衰竭的小鼠中，检测了网状内皮细胞因子葡聚糖的造血作用。在正常小鼠中，给予葡聚糖可增加骨髓和脾脏中可移植的多能造血干细胞（CFU-s）、定向粒细胞-巨噬细胞祖细胞（GM-CFC）以及纯巨噬细胞祖细胞（M-CFC）的含量。红系祖细胞（CFU-e）仅在脾脏中有所增加。在亚致死剂量照射（650拉德）的小鼠中，无论在照射前还是照射后给予葡聚糖，均可增加内源性多能造血干细胞（E-CFU）的数量。在照射前1天、照射前1小时或照射后1小时给予葡聚糖时，观察到的效果最为显著。此外，在致死剂量照射（900拉德）前给予葡聚糖可提高生存率。在照射前1天给予葡聚糖时，观察到的结果最为显著。本文讨论了使用葡聚糖等药物来促进化疗和/或放疗后的造血重建并预防败血症的可能性。
