López-Contreras A M, Claassen P A, Mooibroek H, De Vos W M
Agrotechnological Research Institute (ATO-DLO), Wageningen, The Netherlands.
Appl Microbiol Biotechnol. 2000 Aug;54(2):162-7. doi: 10.1007/s002530000374.
Domestic organic waste (DOW) collected in The Netherlands was analysed and used as substrate for acetone, butanol and ethanol (ABE) production. Two different samples of DOW, referred to as fresh DOW and dried DOW, were treated by extrusion in order to expand the polymer fibres present and to obtain a homogeneous mixture. The extruded material was analysed with respect to solvent and hot water extractives, uronic acids, lignin, sugars and ash. The total sugar content in the polymeric fractions of the materials varied from 27.7% to 39.3% (w/w), in which glucose represented the 18.4 and 25.1% of the materials, for fresh and dried DOW, respectively. The extruded fresh DOW was used as substrate for the ABE fermentation by the solventogenic strain Clostridium acetobutylicum ATCC 824. This strain was grown on a suspension of 10% (w/v) DOW in demineralised water without further nutrient supplement. This strain produced 4 g ABE/100 g extruded DOW. When C. acetobutylicum ATCC 824 was grown on a suspension of 10% (w/v) DOW hydrolysed by a combination of commercial cellulases and beta-glucosidases, the yield of solvents increased to 7.5 g ABE/100 g extruded DOW. The utilisation of sugar polymers in both hydrolysed and non-hydrolysed DOW was determined, showing that only a small proportion of the polymers had been consumed by the bacteria. These results indicate that growth and ABE production on DOW is mainly supported by soluble saccharides in the medium.
对在荷兰收集的家庭有机废物（DOW）进行了分析，并将其用作丙酮、丁醇和乙醇（ABE）生产的底物。两种不同的DOW样品，即新鲜DOW和干燥DOW，通过挤压进行处理，以扩展存在的聚合物纤维并获得均匀混合物。对挤压后的材料进行了溶剂和热水提取物、糖醛酸、木质素、糖和灰分的分析。材料聚合物部分中的总糖含量在27.7%至39.3%（w/w）之间变化，其中葡萄糖分别占新鲜和干燥DOW材料的18.4%和25.1%。挤压后的新鲜DOW用作产溶剂菌株丙酮丁醇梭菌ATCC 824进行ABE发酵的底物。该菌株在去离子水中10%（w/v）DOW的悬浮液中生长，无需进一步补充营养。该菌株产生了4 g ABE/100 g挤压后的DOW。当丙酮丁醇梭菌ATCC 824在由商业纤维素酶和β-葡萄糖苷酶组合水解的10%（w/v）DOW悬浮液中生长时，溶剂产量增加到7.5 g ABE/100 g挤压后的DOW。测定了水解和未水解DOW中糖聚合物的利用率，结果表明只有一小部分聚合物被细菌消耗。这些结果表明，DOW上的生长和ABE生产主要由培养基中的可溶性糖类支持。
