Makler A, Zaidise I, Paldi E, Brandes J M
Fertil Steril. 1979 Feb;31(2):147-54. doi: 10.1016/s0015-0282(16)43815-x.
Semen specimens from 33 donors were analyzed intermittently between 1 and 24 hours after ejaculation. Motility was determined objectively by analyzing still-camera photomicrographs taken with the aid of the new multiple exposure photography (MEP) technique, from which the following information was obtained: the percentage of motile spermatozoa, the average velocity and frequency distribution of spermatozoal velocities, and index of motility as well as sperm concentration. From curves drawn for each of the 33 analyzed specimens, these conclusions were drawn: 1. The percentage of motile spermatozoa decreases progressively, beginning 1 hour after ejaculation, at a rate of about 5% to 10%/hour. 2. In the majority of cases, sperm velocity increases for the first 4 hours and then decreases gradually. 3. There is no unique pattern of sperm motility and velocity with time for all specimens, especially during the first 4 hours. However, three typical curves of motility change were found during the first 4 hours: increase in motility, moderate decline in motility, and rapid loss of motility. 4. The frequency distribution of velocities of motile spermatozoa revealed typical normal curves at all times, but these curves became flatter as time elapsed. An explanation for these divergent patterns and the possible role of these findings in some physiologic and clinical problems are discussed.
对33名捐献者的精液标本在射精后1至24小时内进行了间断分析。通过分析借助新型多重曝光摄影（MEP）技术拍摄的静态相机显微照片客观地确定活力，从中获得了以下信息：活动精子的百分比、精子平均速度和速度频率分布、活力指数以及精子浓度。根据为33个分析标本中的每一个绘制的曲线，得出了以下结论：1. 活动精子的百分比从射精后1小时开始逐渐下降，下降速率约为5%至10%/小时。2. 在大多数情况下，精子速度在最初4小时内增加，然后逐渐下降。3. 并非所有标本的精子活力和速度随时间都有独特模式，尤其是在最初4小时内。然而，在最初4小时内发现了三种典型的活力变化曲线：活力增加、活力适度下降和活力迅速丧失。4. 活动精子的速度频率分布在所有时间都呈现典型的正态曲线，但随着时间推移这些曲线变得更平坦。讨论了这些不同模式的解释以及这些发现在一些生理和临床问题中的可能作用。
