Hosking C S, Pyman C, Wilkins B
Arch Dis Child. 1983 May;58(5):327-9. doi: 10.1136/adc.58.5.327.
We describe a laboratory test which is useful in determining whether deafness is due to intrauterine rubella. The study group consisted of 8 deaf patients with proved intrauterine rubella, 12 patients with familial deafness, and 17 controls. Blood was taken at time 0 from all of them. All the deaf children and 5 controls were immunised and further blood samples taken at 2 and 6 weeks. Each blood sample was analysed for the lymphocyte response to rubella in vitro and for the presence of rubella haemagglutination inhibition. All the rubella-affected patients had antibody titres to rubella at time 0 and there was no notable change after immunisation. None of these patients had a significant lymphocyte response to rubella antigen at any time. Eight of the familial deaf patients and 13 of the controls had positive antibody titres. With the exception of one patient whose results were equivocal, all the familial deaf patients and controls in whom antibodies were detected had lymphocyte responses to rubella. In those with undetectable antibodies the lymphocytes failed to respond in vitro to rubella antigen.
我们描述了一种实验室检测方法，该方法有助于确定耳聋是否由先天性风疹引起。研究组包括8名确诊为先天性风疹的耳聋患者、12名家族性耳聋患者和17名对照者。在时间点0采集了他们所有人的血液样本。所有耳聋儿童和5名对照者接受了免疫接种，并在2周和6周时采集了更多血液样本。对每份血液样本进行体外淋巴细胞对风疹的反应分析以及风疹血凝抑制试验。所有受风疹影响的患者在时间点0时风疹抗体滴度均呈阳性，免疫接种后无明显变化。这些患者在任何时候对风疹抗原均无显著的淋巴细胞反应。8名家族性耳聋患者和13名对照者的抗体滴度呈阳性。除一名患者结果不明确外，所有检测到抗体的家族性耳聋患者和对照者对风疹均有淋巴细胞反应。在那些未检测到抗体的患者中，淋巴细胞在体外对风疹抗原无反应。
