Duarte M I, Sesso A, de Brito T
Am J Pathol. 1978 Jul;92(1):85-98.
Light and electron microscopic studies combined with a morphometric analysis of the hamster glomerulus in experimental kala-azar showed progressive hyperplasia of mesangial cells beginning on the 10th day and reaching a peak on the 20th day after infection. Afterward, the number of mesangial cells declined and a progressive rise of amyloid deposits over the mesangial matrix was observed. This system for amyloid production is unique if we consider that probably one cell, the mesangial cell, is involved in glomerular amyloid deposition. Our data support a slight modification in the sequence of events of the biphasic theory of amyloid formation. We observed that the number of mesangial cells declines when amyloid deposition increases and that mesangial cell morphology in this stage is not that of an actively secreting cell. It is therefore hypothesized that amyloid precursor material is secreted into the matrix during the proliferative phase. In the second phase, amyloid deposits occur in the extracellular media close to functionally impaired mesangial cells.
光学显微镜和电子显微镜研究，结合对实验性黑热病仓鼠肾小球的形态计量分析，结果显示，从感染后第10天开始，系膜细胞逐渐增生，并在第20天达到高峰。此后，系膜细胞数量减少，同时观察到系膜基质上的淀粉样沉积物逐渐增加。如果我们认为可能只有一种细胞，即系膜细胞，参与了肾小球淀粉样沉积，那么这个淀粉样蛋白产生系统是独特的。我们的数据支持对淀粉样蛋白形成双相理论的事件顺序进行轻微修改。我们观察到，当淀粉样沉积增加时，系膜细胞数量减少，且此阶段系膜细胞形态并非活跃分泌细胞的形态。因此，我们推测淀粉样前体物质在增殖期被分泌到基质中。在第二阶段，淀粉样沉积物出现在功能受损的系膜细胞附近的细胞外介质中。
