Suquet C M, Leid R W
Inflammation. 1983 Jun;7(2):197-203. doi: 10.1007/BF00917823.
Platelet-activating factor (PAF), a lipid released as a result of immediate allergic reactions from basophils and mast cells as well as by a variety of other cell types and stimuli, is one of the most potent platelet agonists and hypotensive agents known. Equine platelets stimulated over a wide range of PAF concentrations aggregated in a time- and dose-dependent manner. Maximum aggregation was observed at concentrations of PAF as low as 3.58 x 10(-14) M with platelet-rich plasma (PRP) and 3.58 x 10(-16) M with washed platelets. Furthermore, the aggregation observed did not appear to be breed-dependent. Finally, the platelet arachidonate pathway appeared to play no role in PAF-induced aggregation as exogenous arachidonate did not enhance the reaction, nor were equine platelets pretreated with 38 microM aspirin inhibited in their response to PAF. This level of aspirin totally inhibited the equine platelet aggregation response to arachidonate.
血小板活化因子（PAF）是一种脂质，由嗜碱性粒细胞和肥大细胞在速发型过敏反应中释放，也可由多种其他细胞类型和刺激因素产生。它是已知的最有效的血小板激动剂和降压剂之一。在广泛的PAF浓度范围内刺激马血小板，其聚集呈时间和剂量依赖性。在富含血小板血浆（PRP）中，PAF浓度低至3.58×10⁻¹⁴ M时观察到最大聚集，在洗涤血小板中，PAF浓度低至3.58×10⁻¹⁶ M时观察到最大聚集。此外，观察到的聚集似乎与品种无关。最后，血小板花生四烯酸途径似乎在PAF诱导的聚集中不起作用，因为外源性花生四烯酸不会增强反应，用38 microM阿司匹林预处理的马血小板对PAF的反应也未受到抑制。这个阿司匹林水平完全抑制了马血小板对花生四烯酸的聚集反应。
