Dahlgren C, Stendahl O
Infect Immun. 1982 Jul;37(1):34-9. doi: 10.1128/iai.37.1.34-39.1982.
When polymorphonuclear leukocytes (PMNL) and soluble or particulate matter interact, the cells produce chemiluminescence. The chemotactic peptide formylmethionyl-leucyl-phenylalanine induced a two-peak chemiluminescence response in PMNL. The response was modified, both in magnitude and in the time course of the response, when PMNL were incubated at 22 degrees C for 15 to 120 min before the addition of stimulus. The cellular response to formylmethionyl-leucyl-phenylalanine was also changed by the addition of cell-free supernatants from stored PMNL suspensions to freshly prepared PMNL before the addition of formylmethionyl-leucyl-phenylalanine. This indicated that PMNL during storage released substances that changed the chemiluminescence response. A similar change could be obtained by addition of purified myeloperoxidase to freshly prepared PMNL before the addition of stimulus, indicating that the myeloperoxidase-hydrogen peroxide system can function as a modulator of the cellular response to chemotactic factors.
当多形核白细胞（PMNL）与可溶性或颗粒性物质相互作用时，细胞会产生化学发光现象。趋化肽甲酰甲硫氨酰-亮氨酰-苯丙氨酸可诱导PMNL产生双峰化学发光反应。当在添加刺激物之前将PMNL在22℃下孵育15至120分钟时，该反应在幅度和反应时间进程上均发生了改变。在添加甲酰甲硫氨酰-亮氨酰-苯丙氨酸之前，将储存的PMNL悬浮液的无细胞上清液添加到新制备的PMNL中，也会改变细胞对甲酰甲硫氨酰-亮氨酰-苯丙氨酸的反应。这表明PMNL在储存过程中释放出了改变化学发光反应的物质。在添加刺激物之前，向新制备的PMNL中添加纯化的髓过氧化物酶也可获得类似的变化，这表明髓过氧化物酶-过氧化氢系统可作为细胞对趋化因子反应的调节剂。
