Hornebeck W, Robert L
Adv Exp Med Biol. 1977;79:145-64. doi: 10.1007/978-1-4684-9093-0_14.
Two neutral proteases have been isolated from aortas and human breast tumors. The aortic elastase-like enzyme has been further purified. The details of this purification procedure will be given and some of the properties of the purified enzyme (susceptibility to various kinds of substrates, degree of inhibition of serum inhibitors, alpha 1-antitrypsin and alpha 2-macroglobulin). This elastinolytic activity of the aorta increased with age and with the degree of atherosclerosis. Both parameters seem to act independently and in a cumulative fashion. Elastinolytic activity has been demonstrated in extracts of human breast carcinomas and is exponentially related to the age of the patient. There exists a parallel neosynthesis of elastin which increased also with the age of the patient. Some characteristics of the polymeric elastin isolated from the tumor tissue will be given. The possible role of this neutral protease present in human aortas and human breast carcinomas will be discussed.
已从主动脉和人类乳腺肿瘤中分离出两种中性蛋白酶。类主动脉弹性蛋白酶已得到进一步纯化。将给出该纯化过程的详细信息以及纯化酶的一些特性（对各种底物的敏感性、血清抑制剂、α1 -抗胰蛋白酶和α2 -巨球蛋白的抑制程度）。主动脉的这种弹性蛋白分解活性随年龄和动脉粥样硬化程度的增加而增加。这两个参数似乎相互独立且以累积方式起作用。在人类乳腺癌提取物中已证实存在弹性蛋白分解活性，且与患者年龄呈指数关系。同时存在弹性蛋白的平行新合成，其也随患者年龄增加。将给出从肿瘤组织中分离出的聚合弹性蛋白的一些特性。将讨论这种存在于人类主动脉和人类乳腺癌中的中性蛋白酶的可能作用。
