源自人移行细胞癌的细胞系的超微结构、核型分析及免疫学研究

O'Toole C, Price Z H, Ohnuki Y, Unsgaard B

Br J Cancer. 1978 Jul;38(1):64-76. doi: 10.1038/bjc.1978.164.

A cell line (J82) was derived from a poorly differentiated, invasive, transitional-cell carcinoma, Stage T3. The cells have been propagated in vitro for 5 years and showed 100% aneuploidy and a mixed epithelial-fibroblastic morphology. The majority of cells contained 2Y chromosomes and several distinctive markers. Peripheral-blood lymphocytes from the donor of the J82 cells were tested sequentially for cytotoxicity toward autologous and allogeneic tumour cells. Autologous cytotoxicity was detected against J82 cells in early in vitro passage. Allogeneic lymphocytes from some patients with transitional-cell carcinoma were also cytotoxic to J82 cells in primary culture. However, selective cytotoxicity by lymphoid cells from bladder-carcinoma patients was not detected against J82 cells in long-term tissue culture.

一种细胞系（J82）源自一名T3期低分化浸润性移行细胞癌患者。这些细胞已在体外传代培养5年，显示出100%的非整倍体特征以及上皮-成纤维细胞混合形态。大多数细胞含有两条Y染色体以及几个独特的标志物。对J82细胞供体的外周血淋巴细胞针对自体和异体肿瘤细胞的细胞毒性进行了连续检测。在体外传代早期检测到了针对J82细胞的自体细胞毒性。一些移行细胞癌患者的异体淋巴细胞对原代培养中的J82细胞也具有细胞毒性。然而，未检测到膀胱癌患者的淋巴细胞对长期组织培养中的J82细胞具有选择性细胞毒性。