Brandes J M, Roth E F, Berk P D, Bottone E, Milano C T, Sarkozi L, Zaroulis C G
Transfusion. 1983 Jul-Aug;23(4):325-7. doi: 10.1046/j.1537-2995.1983.23483276868.x.
High-risk premature infants require red cell transfusions for anemia. Placental blood for autologous transfusions can be collected sterilely into citrate-phosphate-dextrose and stored at 4 degrees C. During storage for 8 days, the placental red cell content of adenosine triphosphate remained normal. The 2,3,-diphosphoglycerate concentration of cells stored 8 days declined sharply; however, the P50 value of the oxyhemoglobin dissociation curve declined to 24.4 +/- 2.40 torr. During storage, placental blood underwent an exchange of extracellular Na+ and K+, but no change in glutathione content. Hemolysis was less than 1 percent. Bacteriologic and fungal cultures remained sterile. These in vitro studies suggest that human placental blood can be collected safely and preserved effectively for autologous red cell transfusion therapy.
