Bifano E M, Dracker R A, Lorah K, Palit A
Department of Pediatrics, State University of New York Health Science Center, Syracuse 13210.
Pediatr Res. 1994 Jul;36(1 Pt 1):90-4. doi: 10.1203/00006450-199407001-00016.
Placental blood was harvested using a novel collection system into packs containing citrate-phosphate-dextrose-adenine and then stored for 28 d. Before and during storage, sterility, adequacy of anticoagulation, blood chemistry, and red cell viability were assessed weekly. The average volume of blood collected was 65 mL (range 30-110 mL) with a 0.42 hematocrit once diluted in anticoagulant. All blood culture specimens were sterile at collection and during storage. Adequate anticoagulation (activated partial thromboplastin time > 90 s) on the day of collection was achieved in 15 specimens, all less than 65 mL of blood volume. Ten specimens, in blood volumes ranging from 65 to 110 mL, had mean activated partial thromboplastin time values of 65.3 +/- 10.2 s. Activated partial thromboplastin time values for all 25 specimens at 1, 2, 3, and 4 wk storage were > 90 s, presumably because of the decline in labile clotting factors after 24 h of storage. Hematocrit, red cell ATP, and red cell shape were maintained during storage. As expected, red cell 2,3-diphosphoglycerate declined and potassium levels rose significantly but were not different than levels reported for adult cells similarly preserved. Based on our results, it appears that placental blood represents a potential source of autologous transfusion for the sick neonate over the first month of life.
使用一种新型采集系统将胎盘血采集到含有枸橼酸盐 - 磷酸盐 - 葡萄糖 - 腺嘌呤的血袋中，然后储存28天。在储存前和储存期间，每周评估无菌情况、抗凝是否充分、血液化学指标以及红细胞活力。采集的血液平均体积为65毫升（范围30 - 110毫升），在抗凝剂中稀释一次后的血细胞比容为0.42。所有血培养标本在采集时和储存期间均无菌。15份标本在采集当天实现了充分抗凝（活化部分凝血活酶时间>90秒），所有这些标本的血量均少于65毫升。10份血量在65至110毫升之间的标本，其活化部分凝血活酶时间的平均值为65.3±10.2秒。所有25份标本在储存1、2、3和4周时的活化部分凝血活酶时间值均>90秒，推测是因为储存24小时后不稳定凝血因子减少。储存期间血细胞比容、红细胞ATP和红细胞形态得以维持。正如预期的那样，红细胞2,3 - 二磷酸甘油酸下降，钾水平显著上升，但与同样保存的成人细胞报告的水平无差异。根据我们的结果，胎盘血似乎是出生后第一个月患病新生儿自体输血的潜在来源。
