缺血期间停搏兔心脏中的脂肪酸合成

Fatty acid synthesis in the arrested rabbit heart during ischemia.

作者信息

Gercken G, Trotz M

出版信息

Pflugers Arch. 1983 Jun;398(1):69-72. doi: 10.1007/BF00584716.

Abstract

In rabbit hearts arrested by a carnosine-buffered cardioplegic solution the incorporation of [1-14C]acetate into lipids was investigated. After 10-20 and 60 min of ischemia the radioactivities of phospholipids, mono-, di-, and triacylglycerols, acyl-CoA, acylcarnitine, and free fatty acids were determined. In the first period of ischemia mainly acylcarnitine was labelled (ca. 50%) and only 25% of [14C]-activity was found in phospholipids which showed the lowest specific activity of all lipid classes. After 60 min of ischemia the percentage of total radioactivity of acylcarnitine and phospholipids was decreased, whereas that of neutral lipids was increased to more than 50%. During the increase of total radioactivity the relative specific activity of all lipids decreased except that of triacylglycerols. Only fatty acids up to chain lengths of 16 carbon atoms were labelled. Lauric and myristic acid had high specific activities. These results indicated de novo synthesis of fatty acids accumulating in triacylglycerols during ischemia of the arrested heart.

摘要

在由肌肽缓冲的心脏停搏液使心脏停搏的兔心脏中，研究了[1-¹⁴C]乙酸掺入脂质的情况。在缺血10 - 20分钟和60分钟后，测定了磷脂、单酰甘油、二酰甘油、三酰甘油、酰基辅酶A、酰基肉碱和游离脂肪酸的放射性。在缺血的第一阶段，主要是酰基肉碱被标记（约50%），而在磷脂中仅发现25%的[¹⁴C]活性，磷脂在所有脂质类别中显示出最低的比活性。缺血60分钟后，酰基肉碱和磷脂的总放射性百分比降低，而中性脂质的百分比增加到超过50%。在总放射性增加期间，除三酰甘油外，所有脂质的相对比活性均降低。仅标记了链长至16个碳原子的脂肪酸。月桂酸和肉豆蔻酸具有高比活性。这些结果表明，在停搏心脏缺血期间，三酰甘油中积累的脂肪酸是从头合成的。