Jagiello G, Ducayen M B, Downey R, Jonassen A
Cell Calcium. 1982 May;3(2):153-62. doi: 10.1016/0143-4160(82)90011-2.
Experiments using a Ca2+/Mg2+, serum free media were carried out aimed at clarifying proposed effects of these divalent cations on in vitro meiotic maturation of mouse and cow oocytes. Agents known to perturb intracellular Ca2+ or calmodulin were also studied. Total absence of both cations restricts both oocyte species from completing meiosis I. Media containing Mg2+ and no Ca2+ permitted some maturation in both species. Absence or small amounts of Mg2+ in the media containing control amounts of Ca2+ was much more inhibitory for the cow than the mouse oocyte. Studies of mouse oocyte maturation with Verapamil, Epinephrine and A23187 demonstrated an inhibition of maturation perhaps by the intracellular Ca2+ changes these agents are alleged to induce. A dependency of mouse oocyte maturation on active Ca-Calmodulin complexes was suggested by the calmodulin inhibitor studies.
使用无钙/镁、无血清培养基进行了实验，旨在阐明这些二价阳离子对小鼠和牛卵母细胞体外减数分裂成熟的假定作用。还研究了已知会干扰细胞内钙或钙调蛋白的试剂。两种阳离子完全缺失会限制两种卵母细胞完成减数分裂I。含有镁而无钙的培养基使两种卵母细胞都能实现一定程度的成熟。在含有对照量钙的培养基中，镁的缺失或少量存在对牛卵母细胞的抑制作用比对小鼠卵母细胞大得多。用维拉帕米、肾上腺素和A23187对小鼠卵母细胞成熟进行的研究表明，这些试剂据称会引起细胞内钙变化，从而可能抑制成熟。钙调蛋白抑制剂研究表明，小鼠卵母细胞成熟依赖于活性钙-钙调蛋白复合物。
