Potel J, Arndt K
Arzneimittelforschung. 1982;32(10):1226-33.
Investigations have been carried out in order to clarify the antibiotic susceptibility determination of yeasts. 291 yeast strains of different species were tested for sensitivity to 7 antimycotics: amphotericin B, flucytosin, nystatin, pimaricin, clotrimazol, econazol and miconazol. Additionally to the evaluation of inhibition zone diameters and MIC-values the influence of pH was examined. 1. The dependence of inhibition zone diameters upon pH-values varies due to the antimycotic tested. For standardizing purposes the pH 6.0 is proposed; moreover, further experimental parameters, such as nutrient composition, agar depth, cell density, incubation time and -temperature, have to be normed. 2. The relation between inhibition zone size and logarythmic MIC does not fit a linear regression analysis when all species are considered together. Therefore regression functions have to be calculated selecting the individual species. In case of the antimycotics amphotericin B, nystatin and pimaricin the low scattering of the MIC-values does not allow regression analysis. 3. A quantitative susceptibility determination of yeasts--particularly to the fungistatical substances with systemic applicability, flucytosin and miconazol, -- is advocated by the results of the MIC-tests.
为了阐明酵母对抗生素的敏感性测定，已开展了相关研究。对291株不同种类的酵母菌株进行了7种抗真菌药物敏感性测试，这些抗真菌药物包括两性霉素B、氟胞嘧啶、制霉菌素、匹马霉素、克霉唑、益康唑和咪康唑。除了评估抑菌圈直径和最低抑菌浓度（MIC）值外，还研究了pH值的影响。1. 抑菌圈直径对pH值的依赖性因所测试的抗真菌药物而异。为标准化起见，建议采用pH 6.0；此外，还必须规范其他实验参数，如营养成分、琼脂厚度、细胞密度、孵育时间和温度。2. 当将所有种类的酵母一起考虑时，抑菌圈大小与对数MIC之间的关系不符合线性回归分析。因此，必须选择各个种类来计算回归函数。对于抗真菌药物两性霉素B、制霉菌素和匹马霉素，MIC值的低离散度不允许进行回归分析。3. MIC测试结果提倡对酵母进行定量药敏测定，尤其是对具有全身适用性的抑菌物质氟胞嘧啶和咪康唑。
