Belanger F C, Rebeiz C A
Biochemistry. 1980 Oct 14;19(21):4875-83. doi: 10.1021/bi00562a027.
It is shown that the protochlorophyllide ester pool of etiolated higher plants is a faithful copy of the protochlorophyllide pool. It is made up of both monovinyl- and divinylprotochlorophyllide esters. Although the two tetrapyrroles exhibited similar emission maxima, they were distinguishable by their Soret excitation maxima, which were found at 436-437 and 443-444 nm, respectively, in ether at 77K. The two pigments were partially separated on thin layers of polyethylene. They were accompanied by two unknown fluorescent compounds. It was also shown that during greening, the protochlorophyllide ester pool maintained a constant qualitative composition. This was in sharp contrast with the drastic qualitative changes undergone by the protochlorophyllide pool of etiolated tissues grown under identical conditions.
结果表明，黄化高等植物的原叶绿素酸酯库是原叶绿素酸库的忠实副本。它由单乙烯基和二乙烯基原叶绿素酸酯组成。尽管这两种四吡咯显示出相似的发射最大值，但通过它们在77K的乙醚中的Soret激发最大值可以区分，分别在436 - 437和443 - 444nm处。这两种色素在聚乙烯薄层上部分分离。它们伴随着两种未知的荧光化合物。还表明，在绿化过程中，原叶绿素酸酯库保持恒定的定性组成。这与在相同条件下生长的黄化组织的原叶绿素酸库所经历的剧烈定性变化形成鲜明对比。
