Aonuma S, Okabe M, Kawaguchi M, Kishi Y
J Reprod Fertil. 1981 Nov;63(2):463-6. doi: 10.1530/jrf.0.0630463.
The fertilizing ability of mouse epididymal and capacitated spermatozoa was tested by mixing with normal or zona-free ova. In the presence of Zn2+, the epididymal spermatozoa failed not only to penetrate the zona pellucida but also to fuse with zone-free ova, while no effect was observed on capacitated spermatozoa. Zn2+ also inhibited fertilization with spermatozoa preincubated in Ca2+-free medium. However, shortly after the addition of Ca2+ to the preincubated spermatozoa, Zn2+ lost its fertilization inhibitory activity.
通过与正常或无透明带的卵子混合，检测小鼠附睾精子和获能精子的受精能力。在锌离子存在的情况下，附睾精子不仅无法穿透透明带，也无法与无透明带的卵子融合，而对获能精子则未观察到影响。锌离子还抑制了在无钙离子培养基中预孵育的精子的受精作用。然而，在向预孵育的精子中添加钙离子后不久，锌离子就失去了其受精抑制活性。
