Rozenblatt S, Gesang C, Lavie V, Neumann F S
J Virol. 1982 Jun;42(3):790-7. doi: 10.1128/JVI.42.3.790-797.1982.
Since cloning and characterization of DNA complementary to measles virus mRNA encoding for the nucleocapsid protein (M. Gorecki and S. Rozenblatt, Proc, Natl. Acad, Sci, U.S.A. 77:3686--3690, 1980), two additional measles-specific clones containing different classes of sequences have been characterized. The cloned plasmids contain inserts of 480 and 530 base pairs as shown by agarose gel electrophoresis and electron microscopy. The sizes of the mRNA species complementary to these inserts are 1,700 and 1,550 nucleotides, respectively as determined by the Northern technique. The cloned DNA fragments were further identified as reverse transcripts of the mRNA coding for the glycoprotein and matrix protein of measles virus. The major cell-free translation products of mRNA selected by hybridization to the individual cloned DNAs comigrated with the 70K in vitro products and matrix proteins. One of the cell-free translation products (70K) was also immunoprecipitated specifically with monoclonal antibodies against measles virus glycoprotein.
自从克隆并鉴定出与编码核衣壳蛋白的麻疹病毒信使核糖核酸（mRNA）互补的DNA以来（M. 戈雷茨基和S. 罗森布拉特，《美国国家科学院院刊》77:3686 - 3690, 1980），另外两个含有不同序列类别的麻疹病毒特异性克隆也已得到鉴定。如琼脂糖凝胶电泳和电子显微镜所示，克隆的质粒含有480和530个碱基对的插入片段。通过Northern技术测定，与这些插入片段互补的mRNA种类的大小分别为1700和1550个核苷酸。克隆的DNA片段被进一步鉴定为编码麻疹病毒糖蛋白和基质蛋白的mRNA的逆转录产物。通过与单个克隆DNA杂交选择的mRNA的主要无细胞翻译产物与70K体外产物和基质蛋白一起迁移。其中一种无细胞翻译产物（70K）也被抗麻疹病毒糖蛋白的单克隆抗体特异性免疫沉淀。
