Allen R N
Intervirology. 1982;18(1-2):76-82. doi: 10.1159/000149306.
Molecular hybridization between avocado sunblotch viroid and its complementary DNA (cDNA) proceeds as if it were a bimolecular chemical reaction with a rate constant of 0.21 liters per nanomole of nucleotide per day. Computer simulations show the minimum incubation times required to detect the viroid with cDNA probes of varying sensitivity with respect to viroid concentration, and estimate the concentration of one of the reactants when the other is known.
鳄梨日斑类病毒与其互补DNA(cDNA)之间的分子杂交过程,就如同是一个双分子化学反应,其反应速率常数为每天每纳摩尔核苷酸0.21升。计算机模拟显示了使用对类病毒浓度具有不同灵敏度的cDNA探针检测类病毒所需的最短孵育时间,并在已知另一种反应物浓度时估算其中一种反应物的浓度。