通过与合成寡核苷酸探针杂交检测鳄梨日斑类病毒

Detection of avocado sunblotch viroid by hybridization with synthetic oligonucleotide probes.

作者信息

Bar-Joseph M, Segev D, Twizer S, Rosner A

出版信息

J Virol Methods. 1985 Jan;10(1):69-73. doi: 10.1016/0166-0934(85)90090-4.

Abstract

Two short (20 and 17 nucleotides) DNA hybridization probes, complementary to avocado sunblotch viroid (ASBV) RNA nucleotides 68-87 and 88-104 respectively (Symons, R.H., Nucleic Acid Res. 9, 6527, 1981) were synthesized. The sensitivity and specificity of these radioactively labelled probes for hybridization with RNA of several ASBV isolates are demonstrated.

摘要

合成了两条短的（分别为20和17个核苷酸）DNA杂交探针，它们分别与鳄梨日斑类病毒（ASBV）RNA的核苷酸68 - 87和88 - 104互补（Symons，R.H.，《核酸研究》9，6527，1981）。证明了这些放射性标记探针与几种ASBV分离株RNA杂交的敏感性和特异性。