Lima M I, Fonseca M E, Flores R, Kitajima E W
Departamento de Biologia Celular, Universidade de Brasília, Brazil.
Arch Virol. 1994;138(3-4):385-90. doi: 10.1007/BF01379142.
In situ hybridization experiments were carried out to detect avocado sunblotch viroid (ASBVd) in foliar tissue of avocado, using a digoxigenin-labelled RNA probe complementary to the ASBVd-RNA in sections of aldehyde-fixed, LRGold-embedded leaf samples. Detection of the probe was made through anti-digoxigenin antibody and protein-A colloidal gold (20 nm). Seventy to 80% of the signals came from chloroplast while the cytoplasm and vacuole were labelled with ca. 10% of the gold particles. This is in contrast with the subcellular localization of potato spindle tuber viroid and some other related viroids, which are mainly found in the nucleus.
采用地高辛标记的RNA探针，对醛固定、LRGold包埋的鳄梨叶片样品切片中与鳄梨日斑类病毒（ASBVd）-RNA互补的序列进行原位杂交实验，以检测鳄梨叶片组织中的ASBVd。通过抗地高辛抗体和蛋白A胶体金（20纳米）对探针进行检测。70%至80%的信号来自叶绿体，而细胞质和液泡被约10%的金颗粒标记。这与马铃薯纺锤块茎类病毒和其他一些相关类病毒的亚细胞定位相反，后者主要存在于细胞核中。
