Zighelboim S, Tomasz A
Antimicrob Agents Chemother. 1980 Mar;17(3):434-42. doi: 10.1128/AAC.17.3.434.
Multiply drug-resistant South African pneumococci (with penicillin minimal inhibitory concentrations ranging from 0.2 to 12.5 microgram/ml) showed several types of major alterations in their penicillin-binding protein (PBP) pattern compared with that of a penicillin-susceptible laboratory strain of Streptococcus pneumoniae (R6; penicillin minimal inhibitory concentration = 0.006 microgram/ml). Genetic transformants were obtained by using South African pneumococcus (strain 8249) deoxyribonucleic acid as donor and the competent cells of strain R6 as recipient; seven classes of transformants with progressively higher penicillin resistance were isolated, and their PBPs were tested. The PBP patterns exhibited a gradual shift from a pattern similar to that of the recipient to a pattern resembling that of the donor strain as the level of penicillin resistance increased.
多重耐药的南非肺炎球菌（青霉素最低抑菌浓度范围为0.2至12.5微克/毫升）与青霉素敏感的肺炎链球菌实验室菌株（R6；青霉素最低抑菌浓度=0.006微克/毫升）相比，其青霉素结合蛋白（PBP）模式出现了几种主要变化。以南非肺炎球菌（8249株）脱氧核糖核酸为供体，R6株感受态细胞为受体，获得了遗传转化体；分离出七类青霉素耐药性逐渐升高的转化体，并对其PBPs进行了检测。随着青霉素耐药水平的提高，PBP模式呈现出从类似于受体的模式逐渐转变为类似于供体菌株模式的变化。
