Scherzer H, Ward P A
J Immunol. 1978 Sep;121(3):947-52.
Immune complexes consisting of rabbit antibody to bovine serum albumin (BSA) have been made up at 1X, 3X, 6X, 8X, and 20X antigen equivalence. The complement fixing activity of these complexes is inversely proportional to the amount of antigen present in the complexes, and, as expected, solubility of the complexes progressively increases with increasing amounts of antigen. The ability of these complexes to induce acute pulmonary injury and inflammatory responses has been quantitatively assessed. Complexes preformed at antigen equivalence are the most damaging to lung, correlating with their complement fixing activity. When the antigen concentration in the complexes is increased 3 to 6 times beyond the point of equivalence, the phlogistic activity of the complexes drops off rapidly, as demonstrated by a sharp decline in the changes in vascular permeability, hemorrhage, and morphologic evidence of inflammation. These studies provide the first evidence that changing the physicochemical parameters of preformed immune complexes by simply altering the ratio of antigen to antibody can dramatically alter the phlogistic properties of immune complexes for pulmonary tissue.
已制备出由兔抗牛血清白蛋白（BSA）抗体组成的免疫复合物，其抗原当量分别为1倍、3倍、6倍、8倍和20倍。这些复合物的补体固定活性与复合物中存在的抗原量成反比，正如预期的那样，复合物的溶解度随着抗原量的增加而逐渐增加。已对这些复合物诱导急性肺损伤和炎症反应的能力进行了定量评估。在抗原当量下预先形成的复合物对肺的损伤最大，这与其补体固定活性相关。当复合物中的抗原浓度超过当量点增加3至6倍时，复合物的炎症活性迅速下降，这表现为血管通透性变化、出血以及炎症形态学证据的急剧下降。这些研究首次证明，通过简单改变抗原与抗体的比例来改变预先形成的免疫复合物的物理化学参数，可显著改变免疫复合物对肺组织的炎症特性。
