Weiss L, Mayhew E, Rapp D G, Holmes J C
Br J Cancer. 1982 Jan;45(1):44-53. doi: 10.1038/bjc.1982.6.
When injected i.v. into mice, the F10 subline of B16 melanoma cells produced significantly more lung tumours over a 3-week period than cells of the F101.r-6 subline. However, in animals bearing intramuscular tumours produced by these sublines, the high pulmonary-colonization potential of the F10 cells was not realized, and no significant differences in natural pulmonary metastasis formation were observed in animals with untreated primary cancers, even when they progressed to the moribund state. Massage of i.m. tumours derived from the two sublines produced no change in metastasis and no changes in the numbers of cancer cells in the blood detectable by bioassay. In contrast, massage increased metastasis from tumours derived from an invasive BL6 subline and B16 wild-type cells and, in the case of the wild-type, the numbers of circulating cancer cells. In vitro experiments show that blood cells from non-tumour-bearing animals are toxic to both sublines; but less to F10 than to F101.r-6. In addition, after i.v. injection of radiolabelled cells, more of the F10 subline were retained in the lungs of recipients than the F101.r-6. In spite of these apparent metastatic advantages of the F10 subline following intravasation, the incidence of natural metastases from i.m. F10 and F101.r-6 tumours was similar, suggesting that substantially fewer F10 than F101.r-6 cells gained access to the circulation. Thus, the higher colonization potential of the F10 cells was not matched by its intravasation potential, since metastatic efficiency is determined by the least efficient step in the metastatic process.
将B16黑色素瘤细胞的F10亚系经静脉注射到小鼠体内后，在3周时间内产生的肺肿瘤明显多于F101.r - 6亚系的细胞。然而，在由这些亚系产生的肌肉内肿瘤的动物中，F10细胞的高肺定植潜力并未实现，在原发性癌症未经治疗的动物中，即使它们发展到濒死状态，也未观察到自然肺转移形成的显著差异。对源自这两个亚系的肌肉内肿瘤进行按摩，转移情况没有变化，通过生物测定法检测到的血液中癌细胞数量也没有变化。相比之下，按摩会增加源自侵袭性BL6亚系和B16野生型细胞的肿瘤的转移，对于野生型而言，还会增加循环癌细胞的数量。体外实验表明，来自未患肿瘤动物的血细胞对这两个亚系都有毒性；但对F10的毒性比对F101.r - 6的毒性小。此外，经静脉注射放射性标记细胞后，受体肺部保留的F10亚系细胞比F101.r - 6亚系细胞更多。尽管F10亚系在进入血管后具有这些明显的转移优势，但源自肌肉内F10和F101.r - 6肿瘤的自然转移发生率相似，这表明进入循环的F10细胞比F101.r - 6细胞少得多。因此，F10细胞较高的定植潜力与其进入血管的潜力不匹配，因为转移效率取决于转移过程中效率最低的步骤。
