Baer B W, Kornberg R D
Proc Natl Acad Sci U S A. 1980 Apr;77(4):1890-2. doi: 10.1073/pnas.77.4.1890.
A repeating structure of cytoplasmic poly(A)-ribonucleoprotein is revealed by digestion with T2 RNase. A pattern of fragments that are multiples of about 27 residues is obtained. The repeating structure is readily reconstituted from purified poly(A) and cytoplasmic factors. Reconstitution is specific for poly(A), as shown by the lack of competition by poly(G), poly(C), poly(dA), and tRNA. The repeating structure is absent from the nucleus, and so appears to be formed upon transport to the cytoplasm.
用T2核糖核酸酶消化可揭示细胞质聚腺苷酸核糖核蛋白的重复结构。获得了约27个残基倍数的片段模式。这种重复结构很容易从纯化的聚腺苷酸和细胞质因子中重构出来。如聚鸟苷酸、聚胞苷酸、聚脱氧腺苷酸和转运RNA缺乏竞争性所示，重构对聚腺苷酸具有特异性。细胞核中不存在这种重复结构，因此似乎是在转运到细胞质时形成的。
