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黏菌盘基网柄菌基因组中聚脱氧胸苷酸序列的转录

Transcription of polydeoxythymidylate sequences in the genome of the cellular slime mold, Dictyostelium discoideum.

作者信息

Jacobson A, Firtel R A, Lodish H

出版信息

Proc Natl Acad Sci U S A. 1974 May;71(5):1607-11. doi: 10.1073/pnas.71.5.1607.

DOI:10.1073/pnas.71.5.1607
PMID:4525281
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC388285/
Abstract

Messenger RNA of the cellular slime mold, Dictyostelium discoideum, contains about equimolar amounts of two classes of poly(adenylic acid) [poly(A)]; one is about 25 nucleotides long and the second about 100 nucleotides long. At least half of the messenger RNA molecules contain one sequence each of poly(A)(25) and poly(A)(100); both poly(A) sequences are located near the 3' end of messenger RNA, and the kinetics of their appearance on messenger RNA precursor indicates that poly(A)(25) is added before poly(A)(100). Dictyostelium nuclear DNA contains 14,000-15,000 sequences of poly(dT)(25) which could code for the smaller poly(A) residues. The poly(A)(100) must be added post-transcriptionally. The poly(dT)(25) sequences are interspersed throughout the genome and may well represent transcription termination regions.

摘要

细胞黏菌盘基网柄菌的信使核糖核酸(mRNA)含有两类大致等摩尔量的聚腺苷酸[poly(A)];一类约25个核苷酸长,另一类约100个核苷酸长。至少一半的信使核糖核酸分子各自含有一个聚腺苷酸(25)序列和一个聚腺苷酸(100)序列;这两个聚腺苷酸序列都位于信使核糖核酸的3'端附近,它们在信使核糖核酸前体上出现的动力学表明聚腺苷酸(25)在聚腺苷酸(100)之前添加。盘基网柄菌核DNA含有14,000 - 15,000个聚脱氧胸苷酸(25)序列,这些序列可能编码较小的聚腺苷酸残基。聚腺苷酸(100)必须在转录后添加。聚脱氧胸苷酸(25)序列散布于整个基因组中,很可能代表转录终止区域。

相似文献

1
Transcription of polydeoxythymidylate sequences in the genome of the cellular slime mold, Dictyostelium discoideum.黏菌盘基网柄菌基因组中聚脱氧胸苷酸序列的转录
Proc Natl Acad Sci U S A. 1974 May;71(5):1607-11. doi: 10.1073/pnas.71.5.1607.
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Synthesis of messenger and ribosomal RNA precursors in isolated nuclei of the cellular slime mold Dictyostelium discoideum.在细胞黏菌盘基网柄菌的分离细胞核中信使核糖核酸和核糖体核糖核酸前体的合成
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Transcription and structure of the genome of the cellular slime mold Dictyostelium discoideum.细胞黏菌盘基网柄菌基因组的转录与结构
Cold Spring Harb Symp Quant Biol. 1974;38:899-914. doi: 10.1101/sqb.1974.038.01.092.

引用本文的文献

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A post-transcriptional regulon controlled by TtpA, the single tristetraprolin family member expressed in Dictyostelium discoideum.由 TtpA 控制的转录后调控因子,TtpA 是在盘基网柄菌中表达的单一 tristetraprolin 家族成员。
Nucleic Acids Res. 2021 Nov 18;49(20):11920-11937. doi: 10.1093/nar/gkab983.
2
Fluctuation in polyadenylate size and content in exponential- and stationary-phase cells of Saccharomyces cerevisiae.酿酒酵母指数生长期和稳定期细胞中聚腺苷酸大小和含量的波动
J Bacteriol. 1980 Oct;144(1):74-81. doi: 10.1128/jb.144.1.74-81.1980.
3
Repeating structure of cytoplasmic poly(A)-ribonucleoprotein.细胞质聚腺苷酸化核糖核蛋白的重复结构
Proc Natl Acad Sci U S A. 1980 Apr;77(4):1890-2. doi: 10.1073/pnas.77.4.1890.
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Sequence organization in Dictyostelium: unique structure at the 5'-ends of protein coding genes.盘基网柄菌中的序列组织:蛋白质编码基因5'端的独特结构
Nucleic Acids Res. 1983 Jan 25;11(2):541-52. doi: 10.1093/nar/11.2.541.
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The binding of poly (rA) and poly (rU) to denatured DNA. II. Studies with natural DNAs.聚(rA)和聚(rU)与变性DNA的结合。II. 天然DNA的研究。
Nucleic Acids Res. 1976 Apr;3(4):1029-51. doi: 10.1093/nar/3.4.1029.
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The binding of poly(rA) and poly(rU) to denatured DNA. I. Model studies with homopolymers.聚(rA)和聚(rU)与变性DNA的结合。I. 同聚物的模型研究。
Nucleic Acids Res. 1976 Apr;3(4):1013-27. doi: 10.1093/nar/3.4.1013.
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本文引用的文献

1
RNA in cytoplasmic and nuclear fractions of cellular slime mold amebas.细胞黏菌变形虫细胞质和细胞核组分中的RNA
J Cell Biol. 1970 May;45(2):399-407. doi: 10.1083/jcb.45.2.399.
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Growth of myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture.细胞黏菌盘基网柄菌的黏变形体在无菌培养中的生长。
Biochem J. 1970 Sep;119(2):171-4. doi: 10.1042/bj1190171.
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Thermal transitions in mixtures of polydeoxyribodinucleotides.聚脱氧核糖二核苷酸混合物中的热转变
Biopolymers. 1970;9(9):1105-17. doi: 10.1002/bip.1970.360090911.
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Electrophoretic separation of viral nucleic acids on polyacrylamide gels.病毒核酸在聚丙烯酰胺凝胶上的电泳分离。
J Mol Biol. 1967 Jun 28;26(3):373-87. doi: 10.1016/0022-2836(67)90310-5.
5
Physical and chemical characterization of two- and three-stranded adenine-thymine and adenine-uracil homopolymer complexes.双链和三链腺嘌呤-胸腺嘧啶以及腺嘌呤-尿嘧啶同聚物复合物的物理和化学特性
J Mol Biol. 1966 Sep;20(2):359-89. doi: 10.1016/0022-2836(66)90069-6.
6
Addition of polyadenylate sequences to virus-specific RNA during adenovirus replication.在腺病毒复制过程中,向病毒特异性RNA添加聚腺苷酸序列。
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2806-9. doi: 10.1073/pnas.68.11.2806.
7
Polyadenylic acid sequences in the heterogeneous nuclear RNA and rapidly-labeled polyribosomal RNA of HeLa cells: possible evidence for a precursor relationship.海拉细胞异质性核RNA和快速标记的多核糖体RNA中的聚腺苷酸序列:前体关系的可能证据。
Proc Natl Acad Sci U S A. 1971 Jun;68(6):1336-40. doi: 10.1073/pnas.68.6.1336.
8
A polynucleotide segment rich in adenylic acid in the rapidly-labeled polyribosomal RNA component of mouse sarcoma 180 ascites cells.在小鼠肉瘤180腹水细胞快速标记的多核糖体RNA组分中富含腺苷酸的多核苷酸片段。
Proc Natl Acad Sci U S A. 1971 Jun;68(6):1331-5. doi: 10.1073/pnas.68.6.1331.
9
An adenylic acid-rich sequence in messenger RNA of HeLa cells and its possible relationship to reiterated sites in DNA.海拉细胞信使核糖核酸中富含腺苷酸的序列及其与DNA中重复位点的可能关系。
Proc Natl Acad Sci U S A. 1971 Jun;68(6):1321-5. doi: 10.1073/pnas.68.6.1321.
10
Messenger and heterogeneous nuclear RNA in HeLa cells: differential inhibition by cordycepin.HeLa细胞中的信使核糖核酸和不均一核核糖核酸:虫草素的差异抑制作用
Proc Natl Acad Sci U S A. 1970 Dec;67(4):1878-85. doi: 10.1073/pnas.67.4.1878.