Pandey R S, Kanungo M S
Mol Biol Rep. 1984 Jan;9(4):245-51. doi: 10.1007/BF00775356.
Conformational changes in the chromatin of skeletal muscle of 3-, 14- and 30 day-old developing rats have been studied using DNase I and micrococcal nuclease (MCN). Purified nuclei were digested separately by MCN and DNase I. The rate and extent of digestion by MCN decreases gradually as development proceeds. The electrophoretic pattern of MCN digested DNA, however, shows no change. The kinetics of digestion of nuclei by DNase I show no change with development. However, the electrophoretic pattern of DNase I digested DNA shows a gradual decrease in the amount of 10-30 bp fragments with progressive development. These studies show that the chromatin of the skeletal muscle undergoes certain conformational changes during postnatal development, and such changes in chromatin may be necessary for terminal differentiation of this tissue.
利用脱氧核糖核酸酶I（DNase I）和微球菌核酸酶（MCN）研究了3日龄、14日龄和30日龄发育中大鼠骨骼肌染色质的构象变化。纯化的细胞核分别用MCN和DNase I进行消化。随着发育的进行，MCN消化的速率和程度逐渐降低。然而，MCN消化DNA的电泳图谱没有变化。DNase I消化细胞核的动力学随发育没有变化。但是，DNase I消化DNA的电泳图谱显示，随着发育进程，10 - 30 bp片段的数量逐渐减少。这些研究表明，骨骼肌染色质在出生后发育过程中会发生某些构象变化，而染色质的这种变化可能是该组织终末分化所必需的。
