Matsuda K, Yamanaka Y, Shen C T, Mori M
J Periodontol. 1980 Jul;51(7):400-7. doi: 10.1902/jop.1980.51.7.400.
Plaque-induced inflammation has been documented from various aspects, but is still not well understood. In an effort to clarify the physicochemical properties of dental plaque and to isolate the active factor which give rise to inflammation, we studied a factor in plaque which may be a direct and local cause of gingival inflammation in ODU rats. Rat dental plaque was dissolved in physiologic saline. After being homogenized, the solution was centrifuged and the supernatant (RP) was used as a sample. Protein component (pRP) of PR was isolated by ammonium sulfate fractionated procedure and then deproteinized RP (dpRP) was obtained by trichloroacetic acid treatment. The vascular permeability factor existed in RP and it was affected by heating and pH. Changes in vascular permeability were directly proportionate to the logarithm of the concentration of protein fraction in dental plaque, and were of a delayed type. It was surmised that pRP contained in RP was the main vascular permeability factor, and it was purified by gel filtration on Sephadex G-100 and G-200.
菌斑诱导的炎症已从多个方面得到证实，但仍未被充分理解。为了阐明牙菌斑的物理化学性质并分离出引发炎症的活性因子，我们研究了一种可能是ODU大鼠牙龈炎症直接局部原因的菌斑因子。将大鼠牙菌斑溶解于生理盐水中。匀浆后，溶液进行离心，上清液（RP）用作样本。通过硫酸铵分级分离程序分离出PR的蛋白质成分（pRP），然后通过三氯乙酸处理获得去蛋白化的RP（dpRP）。血管通透性因子存在于RP中，且受热和pH影响。血管通透性的变化与牙菌斑中蛋白质组分浓度的对数成正比，且为迟发型。推测RP中含有的pRP是主要的血管通透性因子，并通过在Sephadex G - 100和G - 200上进行凝胶过滤将其纯化。
