乳糖阻遏蛋白氨基末端DNA结合结构域与聚[d(AT)]复合物形成的光化学诱导动态核极化研究
Photochemically induced dynamic nuclear polarization investigation of complex formation of the NH2-terminal DNA-binding domain of lac repressor with poly[d(AT)].
作者信息
Buck F, Rüterjans H, Kaptein R, Beyreuther K
出版信息
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5145-8. doi: 10.1073/pnas.77.9.5145.
Abstract
The interaction of the NH2-terminal DNA-binding domain of lac repressor with synthetic oligo[d(AT)] was studied by a photo-CIDNP technique (CIDNP is chemically induced dynamic nuclear polarization). Three of the four tyrosines of the NH2-terminal region were found to be accessible to the photosensitive dye. The corresponding ring proton resonances were enhanced in the photo-CIDNP 1H NMR spectrum, and the only histidine (histidine 29) was located at the surface of the domain, which is supposed to be linked to the core protein of lac repressor by a flexible hinge region. After complex formation of the NH2-terminal region with oligo[d(AT)], two of the three tyrosine residues were no longer accessible to solvent or to photosensitive dye, which is strong evidence that the two tyrosines are part of the contact region.
摘要
利用光化学诱导动态核极化技术（CIDNP）研究了乳糖阻遏物氨基末端DNA结合结构域与合成寡聚[d(AT)]的相互作用。发现氨基末端区域的四个酪氨酸中有三个可与光敏染料接触。在光化学诱导动态核极化1H NMR谱中，相应的环质子共振增强，且唯一的组氨酸（组氨酸29）位于该结构域表面，推测其通过一个柔性铰链区与乳糖阻遏物的核心蛋白相连。氨基末端区域与寡聚[d(AT)]形成复合物后，三个酪氨酸残基中的两个不再能接触到溶剂或光敏染料，这有力地证明这两个酪氨酸是接触区域的一部分。
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