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1
Photochemically induced dynamic nuclear polarization investigation of complex formation of the NH2-terminal DNA-binding domain of lac repressor with poly[d(AT)].乳糖阻遏蛋白氨基末端DNA结合结构域与聚[d(AT)]复合物形成的光化学诱导动态核极化研究
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5145-8. doi: 10.1073/pnas.77.9.5145.
2
Photo-CIDNP study of the interaction between lac repressor headpiece and lac operator DNA.乳糖阻遏蛋白头部片段与乳糖操纵基因DNA相互作用的光化学诱导动态核极化研究
FEBS Lett. 1988 Oct 24;239(1):99-104. doi: 10.1016/0014-5793(88)80553-2.
3
Photochemically induced dynamic nuclear polarization NMR study of yeast and horse muscle phosphoglycerate kinase.酵母和马肌肉磷酸甘油酸激酶的光化学诱导动态核极化核磁共振研究
Biochemistry. 1986 Jul 1;25(13):3788-96. doi: 10.1021/bi00361a009.
4
lac repressor-lac operator interaction: NMR observations.乳糖阻遏蛋白-乳糖操纵子相互作用：核磁共振观察结果。
Proc Natl Acad Sci U S A. 1982 Jan;79(2):218-22. doi: 10.1073/pnas.79.2.218.
5
Surface accessibility of aromatic residues in human lysozyme using photochemically induced dynamic nuclear polarization NMR spectroscopy.利用光化学诱导动态核极化核磁共振光谱法研究人溶菌酶中芳香族残基的表面可及性。
FEBS Lett. 1985 Jun 17;185(2):248-52. doi: 10.1016/0014-5793(85)80916-9.
6
Photo CIDNP study on the complex formation of lambda cro protein with OR3.λ阻遏蛋白与OR3复合物形成的光化学诱导动态核极化研究
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7
Exposed tyrosine residues of lambda cro repressor protein evidenced by nitration and photo CIDNP experiments.通过硝化和光化学诱导动态核极化（photo CIDNP）实验证实的λcro阻遏蛋白的暴露酪氨酸残基
J Biochem. 1985 Sep;98(3):799-805. doi: 10.1093/oxfordjournals.jbchem.a135337.
8
NMR study of the interaction between the lac repressor and the lac operator.乳糖阻遏物与乳糖操纵基因相互作用的核磁共振研究
Eur J Biochem. 1983 May 2;132(2):321-7. doi: 10.1111/j.1432-1033.1983.tb07365.x.
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A photo-CIDNP study of the interaction of oligonucleotides with gene-5 protein of bacteriophage M13.噬菌体M13寡核苷酸与基因5蛋白相互作用的光化学诱导动态核极化研究
Proc Natl Acad Sci U S A. 1978 Nov;75(11):5281-5. doi: 10.1073/pnas.75.11.5281.
10
Determination of the solvent accessibility of specific aromatic residues in gamma-crystallin by photo-CIDNP NMR measurements.通过光化学诱导动态核极化核磁共振测量法测定γ-晶状体蛋白中特定芳香族残基的溶剂可及性
Curr Eye Res. 1984 Jan;3(1):127-35. doi: 10.3109/02713688408997194.

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1
Real-time nuclear magnetic resonance spectroscopy in the study of biomolecular kinetics and dynamics.用于生物分子动力学研究的实时核磁共振光谱学。
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A novel tri-enzyme system in combination with laser-driven NMR enables efficient nuclear polarization of biomolecules in solution.一种新型的三酶体系与激光驱动的 NMR 相结合，可实现溶液中生物分子的高效核极化。
J Phys Chem B. 2013 May 23;117(20):6069-81. doi: 10.1021/jp4010168. Epub 2013 Apr 30.
3
lac repressor-lac operator interaction: NMR observations.乳糖阻遏蛋白-乳糖操纵子相互作用：核磁共振观察结果。
Proc Natl Acad Sci U S A. 1982 Jan;79(2):218-22. doi: 10.1073/pnas.79.2.218.
4
Interaction between the lac operator and the lac repressor headpiece: fluorescence and circular dichroism studies.乳糖操纵基因与乳糖阻遏蛋白头部结构域之间的相互作用：荧光和圆二色性研究
EMBO J. 1982;1(11):1405-9. doi: 10.1002/j.1460-2075.1982.tb01330.x.
5
Secondary structure of the lac repressor DNA-binding domain by two-dimensional 1H nuclear magnetic resonance in solution.溶液中二维¹H核磁共振法测定乳糖阻遏蛋白DNA结合结构域的二级结构
Proc Natl Acad Sci U S A. 1983 Oct;80(19):5837-41. doi: 10.1073/pnas.80.19.5837.
6
Evidence for a contact between glutamine-18 of lac repressor and base pair 7 of lac operator.乳糖阻遏蛋白的谷氨酰胺-18与乳糖操纵基因的碱基对7之间存在接触的证据。
Proc Natl Acad Sci U S A. 1986 Jan;83(2):303-7. doi: 10.1073/pnas.83.2.303.
7
Base sequence-specific interactions of operator DNA fragments with the lambda-cro repressor coupled with changes in their conformations.操纵基因DNA片段与λ-cro阻遏物的碱基序列特异性相互作用及其构象变化。
EMBO J. 1987 Apr;6(4):1129-35. doi: 10.1002/j.1460-2075.1987.tb04868.x.
8
Photo-CIDNP study of the interaction between the glucocorticoid receptor DNA-binding domain and glucocorticoid response elements.糖皮质激素受体DNA结合结构域与糖皮质激素反应元件相互作用的光化学诱导动态核极化研究
J Biomol NMR. 1991 May;1(1):105-10. doi: 10.1007/BF01874574.

本文引用的文献

1
An amino-terminal fragment of lac repressor binds specifically to lac operator.乳糖阻遏物的氨基端片段特异性结合乳糖操纵基因。
Proc Natl Acad Sci U S A. 1978 Dec;75(12):5851-4. doi: 10.1073/pnas.75.12.5851.
2
The amino-acid sequence of lac repressor.乳糖阻遏蛋白的氨基酸序列。
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3576-80. doi: 10.1073/pnas.70.12.3576.
3
Removal of proteases from DNase I by chromatography over agarose with covalently attached lima bean protease inhibitor.通过在共价连接利马豆蛋白酶抑制剂的琼脂糖上进行层析从脱氧核糖核酸酶I中去除蛋白酶。
Anal Biochem. 1974 Nov;62(1):180-7. doi: 10.1016/0003-2697(74)90379-0.
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Iodination of Escherichia coli lac repressor. Effect of tyrosine modification on repressor activity.大肠杆菌乳糖阻遏物的碘化作用。酪氨酸修饰对阻遏物活性的影响。
Biochemistry. 1975 Jun 3;14(11):2512-20. doi: 10.1021/bi00682a034.
5
Nuclear magnetic resonance studies of the helix-coil transition of poly (dA-dT) in aqueous solution.水溶液中聚（dA-dT）螺旋-线圈转变的核磁共振研究。
Proc Natl Acad Sci U S A. 1976 Mar;73(3):674-8. doi: 10.1073/pnas.73.3.674.
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Lac repressor and lac operator.乳糖阻遏蛋白与乳糖操纵基因
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Repressors.阻遏物
Adv Protein Chem. 1976;30:1-99. doi: 10.1016/s0065-3233(08)60478-7.
8
A defined molecular-weight distribution of deoxyribonucleic acid after extensive sonication.广泛超声处理后脱氧核糖核酸的特定分子量分布。
Biochem J. 1978 Jul 1;173(1):179-83. doi: 10.1042/bj1730179.
9
Laser photo-CIDNP as a surface probe for proteins in solution.激光光化学诱导动态核极化作为溶液中蛋白质的表面探针。
Nature. 1978 Jul 20;274(5668):293-4. doi: 10.1038/274293a0.
10
Differential mobility of the N-terminal headpiece in the lac-repressor protein.乳糖阻遏蛋白中N端头部结构域的差异迁移率。
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乳糖阻遏蛋白氨基末端DNA结合结构域与聚[d(AT)]复合物形成的光化学诱导动态核极化研究

Photochemically induced dynamic nuclear polarization investigation of complex formation of the NH2-terminal DNA-binding domain of lac repressor with poly[d(AT)].

作者信息

Buck F, Rüterjans H, Kaptein R, Beyreuther K

出版信息

Proc Natl Acad Sci U S A. 1980 Sep;77(9):5145-8. doi: 10.1073/pnas.77.9.5145.

DOI:10.1073/pnas.77.9.5145
PMID:6933550
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC350013/
Abstract

The interaction of the NH2-terminal DNA-binding domain of lac repressor with synthetic oligo[d(AT)] was studied by a photo-CIDNP technique (CIDNP is chemically induced dynamic nuclear polarization). Three of the four tyrosines of the NH2-terminal region were found to be accessible to the photosensitive dye. The corresponding ring proton resonances were enhanced in the photo-CIDNP 1H NMR spectrum, and the only histidine (histidine 29) was located at the surface of the domain, which is supposed to be linked to the core protein of lac repressor by a flexible hinge region. After complex formation of the NH2-terminal region with oligo[d(AT)], two of the three tyrosine residues were no longer accessible to solvent or to photosensitive dye, which is strong evidence that the two tyrosines are part of the contact region.

摘要

利用光化学诱导动态核极化技术（CIDNP）研究了乳糖阻遏物氨基末端DNA结合结构域与合成寡聚[d(AT)]的相互作用。发现氨基末端区域的四个酪氨酸中有三个可与光敏染料接触。在光化学诱导动态核极化1H NMR谱中，相应的环质子共振增强，且唯一的组氨酸（组氨酸29）位于该结构域表面，推测其通过一个柔性铰链区与乳糖阻遏物的核心蛋白相连。氨基末端区域与寡聚[d(AT)]形成复合物后，三个酪氨酸残基中的两个不再能接触到溶剂或光敏染料，这有力地证明这两个酪氨酸是接触区域的一部分。