乳糖阻遏蛋白的谷氨酰胺-18与乳糖操纵基因的碱基对7之间存在接触的证据。
Evidence for a contact between glutamine-18 of lac repressor and base pair 7 of lac operator.
作者信息
Ebright R H
出版信息
Proc Natl Acad Sci U S A. 1986 Jan;83(2):303-7. doi: 10.1073/pnas.83.2.303.
Abstract
Glutamine-18 of the lac repressor (lacR) has been substituted by glycine, by serine, and by leucine. The specificities of wild-type lacR and of the three substituted lacR variants have been analyzed with respect to base pairs 5, 6, 7, 8, 9, and 10 of the lac operator (lacO). The data indicate that [Gly18]lacR, [Ser18]lacR, and [Leu18]lacR lose the ability to distinguish between the O+ base pair G . C and the Oc base pairs T . A and A . T at position 7 of lacO (KdOc/KdO+ approximately equal to 1). In contrast, the three substituted variants retain the ability to discriminate O+ from Oc at each other position, by factors of 9 to 37. Therefore, I propose that glutamine-18 contacts base pair 7 of lacO. These data suggest that the interaction between the helix-turn-helix motif and DNA may be very similar or identical in lacR and the catabolite gene activator protein.
摘要
乳糖阻遏蛋白（lacR）的谷氨酰胺18已被甘氨酸、丝氨酸和亮氨酸取代。已针对乳糖操纵基因（lacO）的碱基对5、6、7、8、9和10分析了野生型lacR以及三种取代型lacR变体的特异性。数据表明，[甘氨酸18]lacR、[丝氨酸18]lacR和[亮氨酸18]lacR失去了区分lacO第7位的O⁺碱基对G.C与Oc碱基对T.A和A.T的能力（KdOc/KdO⁺约等于1）。相比之下，这三种取代变体在其他每个位置仍保留区分O⁺和Oc的能力，区分系数为9至37。因此，我提出谷氨酰胺18与lacO的碱基对7接触。这些数据表明，在lacR和分解代谢基因激活蛋白中，螺旋-转角-螺旋基序与DNA之间的相互作用可能非常相似或相同。
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2
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Biochemistry. 1981 Oct 13;20(21):6109-18. doi: 10.1021/bi00524a030.
3
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