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1
Evidence for a contact between glutamine-18 of lac repressor and base pair 7 of lac operator.乳糖阻遏蛋白的谷氨酰胺-18与乳糖操纵基因的碱基对7之间存在接触的证据。
Proc Natl Acad Sci U S A. 1986 Jan;83(2):303-7. doi: 10.1073/pnas.83.2.303.
2
Use of "loss-of-contact" substitutions to identify residues involved in an amino acid-base pair contact: effect of substitution of Gln18 of lac repressor by Gly, Ser, and Leu.使用“失去接触”取代法来鉴定参与氨基酸-碱基对接触的残基：用甘氨酸、丝氨酸和亮氨酸取代乳糖阻遏物的Gln18的效果。
J Biomol Struct Dyn. 1985 Oct;3(2):281-97. doi: 10.1080/07391102.1985.10508417.
3
Dimeric lac repressors exhibit phase-dependent co-operativity.二聚体乳糖阻遏物表现出相位依赖性协同作用。
J Mol Biol. 1998 Dec 11;284(4):851-7. doi: 10.1006/jmbi.1998.2253.
4
Orientation of the Lac repressor DNA binding domain in complex with the left lac operator half site characterized by affinity cleaving.通过亲和切割表征的与左侧乳糖操纵子半位点结合的乳糖阻遏物DNA结合结构域的取向。
Nucleic Acids Res. 1991 Oct 11;19(19):5233-6. doi: 10.1093/nar/19.19.5233.
5
Origin of the asymmetrical contact between lac repressor and lac operator DNA.乳糖阻遏蛋白与乳糖操纵基因DNA之间不对称接触的起源。
J Mol Biol. 1993 Oct 5;233(3):389-99. doi: 10.1006/jmbi.1993.1519.
6
The roles of residues 5 and 9 of the recognition helix of Lac repressor in lac operator binding.乳糖阻遏蛋白识别螺旋中第5位和第9位残基在乳糖操纵基因结合中的作用。
J Mol Biol. 1991 Mar 20;218(2):313-21. doi: 10.1016/0022-2836(91)90714-h.
7
Operator search by mutant Lac repressors.通过突变型乳糖阻遏物进行算子搜索。
J Mol Biol. 1998 May 8;278(3):549-58. doi: 10.1006/jmbi.1998.1729.
8
Base substitution mutants of the lac operator: in vivo and in vitro affinities for lac repressor.乳糖操纵基因的碱基置换突变体：对乳糖阻遏物的体内和体外亲和力
Gene. 1986;50(1-3):123-32. doi: 10.1016/0378-1119(86)90317-3.
9
Strengthening the dimerisation interface of Lac repressor increases its thermostability by 40 deg. C.增强乳糖阻遏物的二聚化界面可将其热稳定性提高40摄氏度。
J Mol Biol. 2000 Jun 9;299(3):805-12. doi: 10.1006/jmbi.2000.3706.
10
The possible roles of residues 79 and 80 of the Trp repressor from Escherichia coli K-12 in trp operator recognition.来自大肠杆菌K-12的色氨酸阻遏物中第79和80位残基在色氨酸操纵基因识别中的可能作用。
Mol Gen Genet. 1995 Jan 20;246(2):180-95. doi: 10.1007/BF00294681.

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1
Structural basis of ECF-σ-factor-dependent transcription initiation.ECF-σ 因子依赖性转录起始的结构基础。
Nat Commun. 2019 Feb 12;10(1):710. doi: 10.1038/s41467-019-08443-3.
2
Data on publications, structural analyses, and queries used to build and utilize the AlloRep database.用于构建和利用AlloRep数据库的出版物、结构分析及查询数据。
Data Brief. 2016 Jul 9;8:948-57. doi: 10.1016/j.dib.2016.07.006. eCollection 2016 Sep.
3
Dissection of recognition determinants of Escherichia coli sigma32 suggests a composite -10 region with an 'extended -10' motif and a core -10 element.大肠杆菌σ32识别决定因素的剖析表明，其具有一个带有“延伸 -10”基序和核心 -10 元件的复合 -10 区域。
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4
Mutational analysis of Escherichia coli sigma28 and its target promoters reveals recognition of a composite -10 region, comprised of an 'extended -10' motif and a core -10 element.大肠杆菌σ28及其靶启动子的突变分析揭示了对一个复合-10区域的识别，该区域由一个“扩展-10”基序和一个核心-10元件组成。
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5
An altered-specificity mutation in a human POU domain demonstrates functional analogy between the POU-specific subdomain and phage lambda repressor.人类POU结构域中的特异性改变突变表明POU特异性亚结构域与噬菌体λ阻遏物之间存在功能相似性。
Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3887-91. doi: 10.1073/pnas.91.9.3887.
6
Operator-constitutive mutations in a DNA sequence recognized by a yeast homeodomain.酵母同源结构域识别的DNA序列中的操纵子组成型突变。
EMBO J. 1994 May 15;13(10):2378-87. doi: 10.1002/j.1460-2075.1994.tb06521.x.
7
Missing contact probing of DNA-protein interactions.DNA-蛋白质相互作用的缺失接触探测
Proc Natl Acad Sci U S A. 1987 Oct;84(19):6673-6. doi: 10.1073/pnas.84.19.6673.
8
Role of glutamic acid-181 in DNA-sequence recognition by the catabolite gene activator protein (CAP) of Escherichia coli: altered DNA-sequence-recognition properties of [Val181]CAP and [Leu181]CAP.谷氨酸-181在大肠杆菌分解代谢基因激活蛋白（CAP）识别DNA序列中的作用：[缬氨酸181]CAP和[亮氨酸181]CAP改变的DNA序列识别特性。
Proc Natl Acad Sci U S A. 1987 Sep;84(17):6083-7. doi: 10.1073/pnas.84.17.6083.
9
The interaction of the recognition helix of lac repressor with lac operator.乳糖阻遏蛋白的识别螺旋与乳糖操纵基因的相互作用。
EMBO J. 1987 Oct;6(10):3145-53. doi: 10.1002/j.1460-2075.1987.tb02625.x.
10
Efficient Tn10 transposition into a DNA insertion hot spot in vivo requires the 5-methyl groups of symmetrically disposed thymines within the hot-spot consensus sequence.在体内，Tn10高效转座至DNA插入热点需要热点共有序列中对称排列的胸腺嘧啶的5-甲基基团。
Proc Natl Acad Sci U S A. 1987 Nov;84(22):7876-80. doi: 10.1073/pnas.84.22.7876.

本文引用的文献

1
lac repressor-lac operator interaction: NMR observations.乳糖阻遏蛋白-乳糖操纵子相互作用：核磁共振观察结果。
Proc Natl Acad Sci U S A. 1982 Jan;79(2):218-22. doi: 10.1073/pnas.79.2.218.
2
lac Repressor: a proton magnetic resonance look at the deoxyribonucleic acid binding fragment.乳糖阻遏蛋白：对脱氧核糖核酸结合片段的质子磁共振研究
Biochemistry. 1981 Oct 13;20(21):6109-18. doi: 10.1021/bi00524a030.
3
Structure of the DNA-binding region of lac repressor inferred from its homology with cro repressor.从乳糖阻遏物与Cro阻遏物的同源性推断出的乳糖阻遏物DNA结合区域的结构。
Proc Natl Acad Sci U S A. 1982 Mar;79(5):1428-32. doi: 10.1073/pnas.79.5.1428.
4
Photochemically induced dynamic nuclear polarization investigation of complex formation of the NH2-terminal DNA-binding domain of lac repressor with poly[d(AT)].乳糖阻遏蛋白氨基末端DNA结合结构域与聚[d(AT)]复合物形成的光化学诱导动态核极化研究
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5145-8. doi: 10.1073/pnas.77.9.5145.
5
Two helix DNA binding motif of CAP found in lac repressor and gal repressor.在乳糖阻遏物和半乳糖阻遏物中发现的CAP的双螺旋DNA结合基序。
Nucleic Acids Res. 1982 Aug 25;10(16):5085-102. doi: 10.1093/nar/10.16.5085.
6
Homology among DNA-binding proteins suggests use of a conserved super-secondary structure.DNA结合蛋白之间的同源性表明存在保守的超二级结构。
Nature. 1982 Jul 29;298(5873):447-51. doi: 10.1038/298447a0.
7
lac Promoter mutations located downstream from the transcription start site.位于转录起始位点下游的乳糖启动子突变。
J Mol Biol. 1980 May 25;139(3):537-49. doi: 10.1016/0022-2836(80)90145-x.
8
The use of double mutants to detect structural changes in the active site of the tyrosyl-tRNA synthetase (Bacillus stearothermophilus).利用双突变体检测嗜热栖热放线菌酪氨酰-tRNA合成酶活性位点的结构变化。
Cell. 1984 Oct;38(3):835-40. doi: 10.1016/0092-8674(84)90278-2.
9
lacZ translation initiation mutations.β-半乳糖苷酶翻译起始突变
J Mol Biol. 1984 Aug 25;177(4):663-83. doi: 10.1016/0022-2836(84)90043-3.
10
Genetic studies of the lac repressor. XII. Amino acid replacements in the DNA binding domain of the Escherichia coli lac repressor.乳糖阻遏物的遗传学研究。十二。大肠杆菌乳糖阻遏物DNA结合结构域中的氨基酸置换
J Mol Biol. 1984 Nov 25;180(1):205-12. doi: 10.1016/0022-2836(84)90438-8.

乳糖阻遏蛋白的谷氨酰胺-18与乳糖操纵基因的碱基对7之间存在接触的证据。

Evidence for a contact between glutamine-18 of lac repressor and base pair 7 of lac operator.

作者信息

Ebright R H

出版信息

Proc Natl Acad Sci U S A. 1986 Jan;83(2):303-7. doi: 10.1073/pnas.83.2.303.

DOI:10.1073/pnas.83.2.303
PMID:3510433
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC322846/
Abstract

Glutamine-18 of the lac repressor (lacR) has been substituted by glycine, by serine, and by leucine. The specificities of wild-type lacR and of the three substituted lacR variants have been analyzed with respect to base pairs 5, 6, 7, 8, 9, and 10 of the lac operator (lacO). The data indicate that [Gly18]lacR, [Ser18]lacR, and [Leu18]lacR lose the ability to distinguish between the O+ base pair G . C and the Oc base pairs T . A and A . T at position 7 of lacO (KdOc/KdO+ approximately equal to 1). In contrast, the three substituted variants retain the ability to discriminate O+ from Oc at each other position, by factors of 9 to 37. Therefore, I propose that glutamine-18 contacts base pair 7 of lacO. These data suggest that the interaction between the helix-turn-helix motif and DNA may be very similar or identical in lacR and the catabolite gene activator protein.

摘要

乳糖阻遏蛋白（lacR）的谷氨酰胺18已被甘氨酸、丝氨酸和亮氨酸取代。已针对乳糖操纵基因（lacO）的碱基对5、6、7、8、9和10分析了野生型lacR以及三种取代型lacR变体的特异性。数据表明，[甘氨酸18]lacR、[丝氨酸18]lacR和[亮氨酸18]lacR失去了区分lacO第7位的O⁺碱基对G.C与Oc碱基对T.A和A.T的能力（KdOc/KdO⁺约等于1）。相比之下，这三种取代变体在其他每个位置仍保留区分O⁺和Oc的能力，区分系数为9至37。因此，我提出谷氨酰胺18与lacO的碱基对7接触。这些数据表明，在lacR和分解代谢基因激活蛋白中，螺旋-转角-螺旋基序与DNA之间的相互作用可能非常相似或相同。