Deb C, Banik S, Paul B, Mukherjee R, Ghosh K
Andrologia. 1980 May-Jun;12(3):276-80. doi: 10.1111/j.1439-0272.1980.tb00629.x.
Testicular steroidogenesis was determined by radioimmuno assay of serum testosterone and histochemical demonstration of the key steroidogenic enzyme (delta 5 3 beta hydroxysteroid dehydrogenase) activity in the Leydig cells after treatment with mitomycin C (mc), (500 microgram/kg, Bwt). In vivo experiments showed mc treatment causes a remarkable fall of serum testosterone level along with significant reduction in the weights of testis and sex accessories. In vitro studies resulted in a diminution in the delta 5 3 beta hydroxysteroid dehydrogenase and glucose-6-phosphate dehydrogenase activity in the section of the testis after addition of mc (100, 200 microgram/ml) in the incubation medium. The consideration of the above findings coroborated to the suggestion that mc reduced testicular steroidogenesis.
通过放射免疫分析法测定血清睾酮，并在丝裂霉素C（500微克/千克体重）处理后，通过组织化学方法显示睾丸间质细胞中关键类固醇生成酶（δ5 3β-羟类固醇脱氢酶）的活性，以此来确定睾丸类固醇生成。体内实验表明，丝裂霉素C处理会导致血清睾酮水平显著下降，同时睾丸和性附属器官的重量也显著减轻。体外研究发现，在孵育培养基中加入丝裂霉素C（100、200微克/毫升）后，睾丸切片中的δ5 3β-羟类固醇脱氢酶和葡萄糖-6-磷酸脱氢酶活性降低。上述研究结果支持了丝裂霉素C降低睾丸类固醇生成的观点。
