Ramjoué H P, Gordon J
J Biol Chem. 1977 Dec 25;252(24):9065-70.
We have set out to establish a nomenclature for ribosomal proteins which transcends, as far as is possible, the species from which the ribosomes were isolated. Initally, we identified individual proteins of rat liver by two-dimensional polyacrylamide gel electrophoresis and comparison with the classification system established by others (Sherton, C.C., and Wool, I.G. (1972) J. Biol. Chem. 247, 4460-4467). This was possible with minor modifications. We carried out a detailed comparison of the rat liver proteins with those of chick liver; parallel separate and mixed preparations of ribosomal subunits from the two species were made. Spots on the two-dimensional gels were carefully scrutinized for possible species differences. Proteins which gave congruent spots for the separate and the mixed preparations were tentatively assumed to be identical between the two species. For these proteins, a species-independent nomenclature was adopted. Contrary to earlier reports, a number of proteins were found which were not identical between the two species. These were assigned a species-specific nomenclature. In all, 7 proteins in the small subunit and 17 from the large subunit of the rat liver ribosome were found to be significantly different in the chick. The evolutionary implications of this are discussed.
我们已着手建立一套核糖体蛋白命名法，该命名法尽可能超越核糖体所分离自的物种。最初，我们通过二维聚丙烯酰胺凝胶电泳鉴定大鼠肝脏中的各个蛋白，并与其他人建立的分类系统进行比较（Sherton, C.C., 和 Wool, I.G. (1972) J. Biol. Chem. 247, 4460 - 4467）。稍作修改后即可实现。我们对大鼠肝脏蛋白与鸡肝脏蛋白进行了详细比较；制备了来自这两个物种的核糖体亚基的平行单独和混合制剂。仔细检查二维凝胶上的斑点以寻找可能的物种差异。对于单独制剂和混合制剂产生一致斑点的蛋白质，初步假定这两个物种中的蛋白质是相同的。对于这些蛋白质，采用了不依赖物种的命名法。与早期报告相反，发现了一些在这两个物种中不相同的蛋白质。这些被赋予了物种特异性命名法。总共发现大鼠肝脏核糖体小亚基中的7种蛋白质和大亚基中的17种蛋白质在鸡中存在显著差异。讨论了其进化意义。
