Kirshner J, Goldberg J
Am J Clin Pathol. 1981 Apr;75(4):617-21. doi: 10.1093/ajcp/75.4.617.
Although acute myeloid leukemia is characterized by defective granulocytic maturation in vivo, blasts from an occasional patient will differentiate in vitro. Leukemic myeloblasts from a 49-year-old woman who had acute myeloid leukemia were suspended in liquid culture containing McCoy's 5A medium with 15% fetal calf serum. Conditioned medium, prepared from normal human peripheral blood adherent cells incubated with 2-mercaptoethanol in McCoy's 5A medium with 15% fetal calf serum, was added to the cultures at a concentration of 40%. Within six days, cellular differentiation to polymorphonuclear granulocytes was observed. These cells were capable of phagocytosis. Attempts to induce differentiation of leukemic cells in liquid culture will, it is hoped, contribute to the understanding of defective cellular maturation in this disease.
尽管急性髓系白血病在体内的特征是粒细胞成熟缺陷，但偶尔有患者的原始细胞在体外会发生分化。一名患有急性髓系白血病的49岁女性的白血病原始粒细胞悬浮于含有15%胎牛血清的 McCoy's 5A 培养基的液体培养物中。将由正常人外周血贴壁细胞在含有15%胎牛血清的 McCoy's 5A 培养基中与2-巯基乙醇一起孵育制备的条件培养基，以40%的浓度添加到培养物中。在六天内，观察到细胞分化为多形核粒细胞。这些细胞能够进行吞噬作用。希望在液体培养中诱导白血病细胞分化的尝试将有助于理解这种疾病中细胞成熟缺陷的问题。
