Tsuda H, Maeda H, Kishimoto S
Br J Cancer. 1981 Jun;43(6):793-803. doi: 10.1038/bjc.1981.117.
Intracellular fluorescence polarization (IFP) values of normal human lymphocytes and leukaemic cells from newly diagnosed patients were determined from fluorescence polarization using fluorescein diacetate (FDA). Thirty healthy donors and 40 patients with various types of leukaemia (20 myelogenous and 20 lymphocytic) were included in the present studies. The result was that myeloid cells had about twice the polarization value of lymphocytic cells. The use of FDA for the determination of IFP appears to be useful for differential diagnosis, at least between acute myelogenous and lymphocytic leukaemias. These 2 types of leukaemia also showed a pronounced difference in fluorescence intensity when treated with FDA, perhaps owing to a difference in uptake velocity. The previously described membrane microviscosity using 1,6-diphenyl-1,3,5-hexatriene (DPH), however, did not show such a difference between these 2 leukaemias. The fluorescein-binding protein(s) was also investigated in order to clarify its effect on IFP, but there seemed little evidence for the existence of any such dyebinding protein(s). The advantages of the present method, using FDA, reside in its simplicity, rapidity and considerable sensitivity, requiring a small sample of blood usually less than 5 ml.
使用荧光素二乙酸酯（FDA）通过荧光偏振法测定了新诊断患者的正常人淋巴细胞和白血病细胞的细胞内荧光偏振（IFP）值。本研究纳入了30名健康供体和40名患有各种类型白血病的患者（20例髓细胞性白血病和20例淋巴细胞性白血病）。结果是髓细胞的偏振值约为淋巴细胞的两倍。使用FDA测定IFP似乎对鉴别诊断有用，至少在急性髓细胞性白血病和淋巴细胞性白血病之间。这两种类型的白血病在用FDA处理时荧光强度也有明显差异，这可能是由于摄取速度不同。然而，先前描述的使用1,6 - 二苯基 - 1,3,5 - 己三烯（DPH）的膜微粘度在这两种白血病之间并未显示出这种差异。还研究了荧光素结合蛋白，以阐明其对IFP的影响，但似乎几乎没有证据表明存在任何此类染料结合蛋白。使用FDA的本方法的优点在于其简单、快速且灵敏度相当高，通常只需不到5毫升的少量血液样本。
